Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export.
Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export.
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DOI：
10.1083/jcb.200106046
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发表时间：
2002-02-18
期刊：
影响因子：
--
通讯作者：
Gerace L
中科院分区：
文献类型：
--
作者：
Frosst P;Guan T;Subauste C;Hahn K;Gerace L
Tpr是在孔复合物的核质侧附近发现的卷曲螺旋蛋白。由于Tpr的精确定位及其功能都没有很好的定义，我们产生了Tpr三个区域的抗体来澄清这些问题。使用光和EM免疫定位，我们确定，哺乳动物Tpr是集中在核篮孔复合物的分布类似Nup 153和Nup 98。抗体定位与活细胞中GFP-Tpr的成像一起揭示，Tpr类似于几种其他核孔蛋白在细胞核内的离散病灶中，但不存在于核内丝状网络中或如所提出的从孔复合物延伸的长丝中。注射抗TPR抗体到有丝分裂细胞中导致TPR从核膜中耗尽，而不损失其他孔复合物篮蛋白。而核进口介导的碱性氨基酸信号不受影响，核出口介导的亮氨酸丰富的信号显着延迟。在间期细胞中的抗Tpr抗体的核注射同样产生蛋白质输出的抑制，但不输入。这些结果表明，Tpr是一个核篮的核孔蛋白，在核蛋白输出的作用。
Tpr is a coiled-coil protein found near the nucleoplasmic side of the pore complex. Since neither the precise localization of Tpr nor its functions are well defined, we generated antibodies to three regions of Tpr to clarify these issues. Using light and EM immunolocalization, we determined that mammalian Tpr is concentrated within the nuclear basket of the pore complex in a distribution similar to Nup153 and Nup98. Antibody localization together with imaging of GFP-Tpr in living cells revealed that Tpr is in discrete foci inside the nucleus similar to several other nucleoporins but is not present in intranuclear filamentous networks or in long filaments extending from the pore complex as proposed. Injection of anti-Tpr antibodies into mitotic cells resulted in depletion of Tpr from the nuclear envelope without loss of other pore complex basket proteins. Whereas nuclear import mediated by a basic amino acid signal was unaffected, nuclear export mediated by a leucine-rich signal was retarded significantly. Nuclear injection of anti-Tpr antibodies in interphase cells similarly yielded inhibition of protein export but not import. These results indicate that Tpr is a nucleoporin of the nuclear basket with a role in nuclear protein export.
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影响因子：
7.8
作者：
CORDES, VC;REIDENBACH, S;FRANKE, WW
通讯作者：
FRANKE, WW
影响因子：
3.7
作者：
Cordes, VC;Hase, ME;Müller, L
通讯作者：
Müller, L
影响因子：
11.4
作者：
Nakielny, S;Shaikh, S;Dreyfuss, G
通讯作者：
Dreyfuss, G
将蛋白质p270/TPR鉴定为核孔复合体核内细丝的组成部分。
DOI：
10.1083/jcb.136.3.515
发表时间：
1997-02-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cordes VC;Reidenbach S;Rackwitz HR;Franke WW
通讯作者：
Franke WW
影响因子：
3
作者：
JARNIK, M;AEBI, U
通讯作者：
AEBI, U