Self reactive repertoire of tight skin mouse: immunochemical and molecular characterization of anti-topoisomerase I autoantibodies.
Self reactive repertoire of tight skin mouse: immunochemical and molecular characterization of anti-topoisomerase I autoantibodies.
复制标题
紧皮小鼠的自身反应谱：抗拓扑异构酶 I 自身抗体的免疫化学和分子特征。
作者：
T. Muryoi;K. Kasturi;M. J. Kafina;Y. Saitoh;O. Usuba;J. S. Perlish;R. Fleischmajer;C. Bona
紧皮（TSK）小鼠发生皮肤增生，伴有皮肤和胶原代谢的组织病理学改变，与人类硬皮病中描述的相似。弥漫性硬皮病是进行性系统性硬化症的最严重形式，与硬皮病70抗原（拓扑异构酶I）特异性自身抗体的产生有关。我们的研究表明，血清抗拓扑异构酶I（topo I）的自身抗体在老年TSK小鼠的水平增加。从TSK小鼠分离的单克隆抗体结合与来自硬皮病患者的自身抗体相互作用的表位。来自老年TSK小鼠的大量TSK单克隆抗拓扑异构酶I抗体和血清免疫球蛋白（IG）具有交叉反应性独特型（Id），该独特型可被从2月龄TSK小鼠获得的同基因单克隆抗Id抗体识别。通过单克隆抗拓扑I抗体对V基因使用的分析表明，这些抗体中的大多数是由来自VHJ 558家族的VH基因编码的，其与来自不同家族的VK基因以随机方式配对。
Tight skin (TSK) mice develop cutaneous hyperplasia accompanied by histopathological alterations of skin and collagen metabolism similar to those described in human scleroderma. Diffuse scleroderma, the most severe form of progressive systemic sclerosis, is associated with the production of autoantibodies specific for Scleroderma 70 antigen (topoisomerase I). Our studies show that there is an increase in the level of serum anti-topoisomerase I (topo I) autoantibodies in aged TSK mice. The monoclonal antibodies isolated from TSK mice bind to epitopes which interact with autoantibodies from scleroderma patients. A significant number of TSK monoclonal anti-topo I antibodies and serum immunoglobulin (Ig) from aged TSK mice bear a cross reactive idiotype (Id) recognized by a syngeneic monoclonal anti-Id antibody obtained from a 2 month-old TSK mouse. Analysis of V gene usage by monoclonal anti-topo I antibodies showed that the majority of these antibodies are encoded by VH genes derived from VHJ558 family pairing with VK genes from various families in a stochastic manner.
紧致皮肤 (TSK) 小鼠成纤维细胞中胶原蛋白生物合成增加，I 型和 III 型前胶原基因表达增加。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jimenez,SA;Williams,CJ;Myers,JC;Bashey,RI
通讯作者：
Bashey,RI
影响因子：
56.9
作者：
SHERO, JH;BORDWELL, B;EARNSHAW, WC
通讯作者：
EARNSHAW, WC
11 种 MRL-lpr/lpr 抗 DNA 自身抗体由来自四个 VH 基因家族的基因编码：VH 基因的使用可能存在偏差。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
TrepicchioJr,W;Barrett,KJ
通讯作者：
Barrett,KJ