Detection of circulating genetically abnormal cells in peripheral blood for early diagnosis of non-small cell lung cancer.
Detection of circulating genetically abnormal cells in peripheral blood for early diagnosis of non-small cell lung cancer.
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检测外周血中循环遗传异常细胞以早期诊断非小细胞肺癌
DOI：
10.1111/1759-7714.13654
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发表时间：
2020-11
期刊：
影响因子：
2.9
通讯作者：
Wang CL
中科院分区：
文献类型：
--
作者：
Liu WR;Zhang B;Chen C;Li Y;Ye X;Tang DJ;Zhang JC;Ma J;Zhou YL;Fan XJ;Yue DS;Li CG;Zhang H;Ma YC;Huo YS;Zhang ZF;He SY;Wang CL
已证实非小细胞肺癌（NSCLC）中存在具有特定染色体变异的循环遗传异常细胞（CAC）。然而，CAC检测的诊断性能仍不清楚。本研究旨在评价CAC检测在NSCLC早期诊断中的潜在临床应用。在这项前瞻性研究中，共招募了339名参与者（261名肺癌患者和78名健康志愿者）。使用抗原非依赖性荧光原位杂交计数外周血中CAC的数量。发现早期NSCLC患者的CAC数量显著高于健康受试者（1.34 vs. 0.19; P < 0.001）。CAC检验显示，区分I期NSCLC和健康受试者的受试者工作特征（ROC）曲线下面积为0.76139，灵敏度和特异性分别为67.2%和80.8%。与血清肿瘤标志物相比，CAC检测区分早期NSCLC的敏感性更高（67.2%vs48.7%，P < 0.001），尤其是小结节的NSCLC患者（65.4% vs. 36.5%，P = 0.003）和磨玻璃样结节（纯GGN：66.7% vs. 40.9%，P = 0.003;混合GGN：73.0% vs. 43.2%，P < 0.001）。CAC检测在早期NSCLC中是可行的。我们的研究表明，CACs可作为一种有前途的非侵入性生物标志物，用于NSCLC的早期诊断。本研究补充：本研究旨在评估CAC检测在NSCLC早期诊断中的潜在临床应用。研究的重要发现：CAC检测在早期NSCLC中是可行的。我们的研究表明，CACs可作为一种有前途的非侵入性生物标志物，用于NSCLC的早期诊断。循环遗传异常细胞计数的工作流程。在早期非小细胞肺癌中进行循环遗传异常细胞检测是可行的。循环遗传异常细胞检测具有良好的稳定性和适应性。循环中的遗传异常细胞可作为非小细胞肺癌早期诊断的一种无创性生物标志物。
Circulating genetically abnormal cells (CACs) with specific chromosome variations have been confirmed to be present in non‐small cell lung cancer (NSCLC). However, the diagnostic performance of CAC detection remains unclear. This study aimed to evaluate the potential clinical application of the CAC test for the early diagnosis of NSCLC. In this prospective study, a total of 339 participants (261 lung cancer patients and 78 healthy volunteers) were enrolled. An antigen‐independent fluorescence in situ hybridization was used to enumerate the number of CACs in peripheral blood. Patients with early‐stage NSCLC were found to have a significantly higher number of CACs than those of healthy participants (1.34 vs. 0.19; P < 0.001). The CAC test displayed an area under the receiver operating characteristic (ROC) curve of 0.76139 for discriminating stage I NSCLC from healthy participants with 67.2% sensitivity and 80.8% specificity, respectively. Compared with serum tumor markers, the sensitivity of CAC assays for distinguishing early‐stage NSCLC was higher (67.2% vs. 48.7%, P < 0.001), especially in NSCLC patients with small nodules (65.4% vs. 36.5%, P = 0.003) and ground‐glass nodules (pure GGNs: 66.7% vs. 40.9%, P = 0.003; mixed GGNs: 73.0% vs. 43.2%, P < 0.001). CAC detection in early stage NSCLC was feasible. Our study showed that CACs could be used as a promising noninvasive biomarker for the early diagnosis of NSCLC. What this study adds: This study aimed to evaluate the potential clinical application of the CAC test for the early diagnosis of NSCLC. Significant findings of the study: CAC detection in early stage NSCLC was feasible. Our study showed that CACs could be used as a promising noninvasive biomarker for the early diagnosis of NSCLC. Workflow of circulating genetically abnormal cells enumeration. Circulating genetically abnormal cell detection in early stage non‐small‐cell lung cancer was feasible. Circulating genetically abnormal cell test has good stability and adaptability for different patient populations. Circulating genetically abnormal cells could be used as a promising noninvasive biomarker for the early diagnosis of NSCLC.
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DOI：
10.1038/nrc.2016.56
发表时间：
2016-08
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
Vargas AJ;Harris CC
通讯作者：
Harris CC
影响因子：
9.7
作者：
Mostert, Bianca;Kraan, Jaco;Sleijfer, Stefan
通讯作者：
Sleijfer, Stefan
DOI：
10.1126/science.1235226
发表时间：
2013-09-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Plaks V;Koopman CD;Werb Z
通讯作者：
Werb Z
影响因子：
20.4
作者：
Goldstraw, Peter;Chansky, Kari;Bolejack, Vanessa
通讯作者：
Bolejack, Vanessa
DOI：
10.1158/1078-0432.ccr-09-3358
发表时间：
2010-08-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Katz RL;He W;Khanna A;Fernandez RL;Zaidi TM;Krebs M;Caraway NP;Zhang HZ;Jiang F;Spitz MR;Blowers DP;Jimenez CA;Mehran RJ;Swisher SG;Roth JA;Morris JS;Etzel CJ;El-Zein R
通讯作者：
El-Zein R