Sex-Dependent Claudin-1 Expression in the Liver of Euthyroid and Hypothyroid Mice
Sex-Dependent Claudin-1 Expression in the Liver of Euthyroid and Hypothyroid Mice
复制标题
甲状腺功能正常和甲状腺功能减退小鼠肝脏中性别依赖性 Claudin-1 表达
DOI：
10.1159/000431316
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发表时间：
2015
影响因子：
4.7
通讯作者：
Führer D
中科院分区：
文献类型：
--
作者：
Zwanziger D;Rakov H;Engels K;Moeller LC;Führer D
背景资料：在肝脏中，紧密连接蛋白claudin-1通过维持胆小管和胆管的细胞旁屏障在胆汁分泌中起重要作用。在甲状腺功能减退症患者中发现胆汁排泄减少，并且甲状腺功能减退症中胆结石的患病率增加。然而，这种关联仅适用于男性，与一般人群中胆道疾病的女性优势形成鲜明对比。目的：我们假设甲状腺功能减退可能导致肝脏中claudin-1表达的改变，并且这种影响可能具有性别特异性。研究方法：我们的特点claudin-1的表达和定位在甲状腺功能正常和甲状腺功能减退的男性和女性C57 BL/6NTac小鼠的肝脏实时PCR，蛋白质印迹和免疫荧光。结果：Claudin-1在小鼠肝小管和胆管中均有表达。雌性小鼠的肝脏表现出较低的claudin-1表达比雄性肝脏。在甲状腺功能减退的肝脏中，雌性动物显示出claudin-1表达升高，而与甲状腺功能正常的对照组相比，在雄性动物中发现claudin-1表达降低。结论：我们证明了小鼠肝脏中claudin-1的表达与甲状腺功能减退症之间的相关性。此外，claudin-1表达的性别依赖性改变被发现。AbstractBackground：在肝脏中，紧密连接蛋白claudin-1在胆汁分泌中起着重要的作用，通过维持胆管和胆小管的细胞旁屏障。在甲状腺功能减退症患者中发现胆汁排泄减少，并且甲状腺功能减退症中胆结石的患病率增加。然而，这种关联仅适用于男性，与一般人群中胆道疾病的女性优势形成鲜明对比。目的：我们假设甲状腺功能减退可能导致肝脏中claudin-1表达的改变，并且这种影响可能具有性别特异性。研究方法：我们的特点claudin-1的表达和定位在甲状腺功能正常和甲状腺功能减退的男性和女性C57 BL/6NTac小鼠的肝脏实时PCR，蛋白质印迹和免疫荧光。结果：Claudin-1在小鼠肝小管和胆管中均有表达。雌性小鼠的肝脏表现出较低的claudin-1表达比雄性肝脏。在甲状腺功能减退的肝脏中，雌性动物显示出claudin-1表达升高，而与甲状腺功能正常的对照组相比，在雄性动物中发现claudin-1表达降低。结论：我们证明了小鼠肝脏中claudin-1的表达与甲状腺功能减退症之间的相关性。此外，claudin-1表达的性别依赖性改变被发现。
Background: In the liver the tight junction protein claudin-1 plays an important role in bile secretion by maintaining the paracellular barrier of bile canaliculi and the bile duct. A diminished bile excretion has been found in hypothyroid patients, and the prevalence of gallstones is increased in hypothyroidism. This association, however, only applies for men and is in contrast to the well-established female preponderance of biliary disease in the general population. Objectives: We hypothesized that hypothyroidism could lead to altered claudin-1 expression in the liver, and that this effect may be sex specific. Methods: We characterized claudin-1 expression and localization in livers of euthyroid and hypothyroid male and female C57BL/6NTac mice by real-time PCR, Western blot and immunofluorescence. Results: Claudin-1 is expressed in canalicular regions and the bile ducts of the murine liver. Livers of female mice showed lower claudin-1 expression than male livers. In hypothyroid livers, female animals showed an elevated claudin-1 expression, whereas reduced claudin-1 expression was found in male animals compared to the euthyroid controls. Conclusion: We demonstrate a correlation between claudin-1 expression and hypothyroidism in the murine liver. Furthermore, a sex-dependent alteration of claudin-1 expression was found.AbstractBackground: In the liver the tight junction protein claudin-1 plays an important role in bile secretion by maintaining the paracellular barrier of bile canaliculi and the bile duct. A diminished bile excretion has been found in hypothyroid patients, and the prevalence of gallstones is increased in hypothyroidism. This association, however, only applies for men and is in contrast to the well-established female preponderance of biliary disease in the general population. Objectives: We hypothesized that hypothyroidism could lead to altered claudin-1 expression in the liver, and that this effect may be sex specific. Methods: We characterized claudin-1 expression and localization in livers of euthyroid and hypothyroid male and female C57BL/6NTac mice by real-time PCR, Western blot and immunofluorescence. Results: Claudin-1 is expressed in canalicular regions and the bile ducts of the murine liver. Livers of female mice showed lower claudin-1 expression than male livers. In hypothyroid livers, female animals showed an elevated claudin-1 expression, whereas reduced claudin-1 expression was found in male animals compared to the euthyroid controls. Conclusion: We demonstrate a correlation between claudin-1 expression and hypothyroidism in the murine liver. Furthermore, a sex-dependent alteration of claudin-1 expression was found.
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影响因子：
1.4
作者：
Jayasingh IA;Puthuran P
通讯作者：
Puthuran P
影响因子：
13.5
作者：
VANSTEENBERGEN, W;FEVERY, J;DEGROOTE, J
通讯作者：
DEGROOTE, J
DOI：
10.1152/ajpendo.00416.2014
发表时间：
2014-12-15
影响因子：
5.1
作者：
Morgan, Daniel H.;Ghribi, Othman;Chen, Xuesong
通讯作者：
Chen, Xuesong
影响因子：
1.9
作者：
Laukkarinen, Johanna;Sand, Juhani;Nordback, Isto
通讯作者：
Nordback, Isto
DOI：
10.1007/s00795-003-0220-5
发表时间：
2003-09-01
期刊：
Medical Electron Microscopy
影响因子：
--
作者：
Kojima, Takashi;Yamamoto, Toshinobu;Sawada, Norimasa
通讯作者：
Sawada, Norimasa