Immunoradiometric studies with monoclonal antibody against a component related to human estrogen receptor.
Immunoradiometric studies with monoclonal antibody against a component related to human estrogen receptor.
复制标题
使用针对人类雌激素受体相关成分的单克隆抗体进行免疫放射研究。
作者：
A. Coffer;G. H. Spiller;K. Lewis;R. King
用从人子宫肌层中提纯的人特异性单抗(D5)检测乳腺肿瘤、子宫肌层、子宫内膜和子宫内膜癌组织胞浆中的反应性抗原，建立了一种简便、快速、灵敏的固相免疫放射分析(IRMA)方法。IRMA在人类乳腺肿瘤和子宫内膜癌的RE阴性细胞液中没有检测到抗原。鸡输卵管、小牛和大鼠子宫的胞浆再阳性不能产生IRMA反应。一项初步研究表明，乳腺肿瘤的D5 IRMA值与[~3H]雌二醇结合部位测定的RE部位有显著相关性(P<0.001)。D5抗原的存在依赖于胞浆RE的存在，而不依赖于孕激素受体的存在。50岁及以上的再阳性患者的D5测定值显著高于50岁以下的患者。这些数据表明，D5抗原是雌激素受体的一种成分，或与该受体在人类细胞中协同调节，该检测方法可能具有临床应用价值。
A human specific monoclonal antibody (D5) raised against a Mr 36,000 cytosolic estrogen receptor component (RE) partially purified from human myometrium was used to develop a simple, rapid, and sensitive solid-phase immunoradiometric assay (IRMA) for the reactive antigen in tissue cytosols from breast tumors, myometrium, endometrium, and endometrial carcinomas. The IRMA did not detect antigen in RE-negative cytosols from human breast tumors and endometrial carcinomas. RE-positive cytosols from chick oviduct and calf and rat uteri failed to produce an IRMA response. A pilot study indicated a significant correlation (P less than 0.001) between D5 IRMA value and RE sites in breast tumors assayed by [3H]estradiol binding sites. The presence of D5 antigen was dependent on the presence of cytosolic RE but not progesterone receptor. RE-positive patients age 50 years and over demonstrated significantly higher D5 assay values than did patients under 50 years. The data suggest that the D5 antigen is a component of the estrogen receptor or coordinately regulated with the receptor in human cells and that the assay method may have clinical use.
影响因子：
2.2
作者：
UOTILA, M;RUOSLAHTI, E;ENGVALL, E
通讯作者：
ENGVALL, E
DOI：
10.1073/pnas.77.9.5115
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GREENE, GL;NOLAN, C;JENSEN, EV
通讯作者：
JENSEN, EV