Luteolin Isolated from Polygonum cuspidatum Is a Potential Compound against Nasopharyngeal Carcinoma.
Luteolin Isolated from Polygonum cuspidatum Is a Potential Compound against Nasopharyngeal Carcinoma.
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从虎杖中分离出的木犀草素是一种潜在的抗鼻咽癌化合物
DOI：
10.1155/2022/9740066
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发表时间：
2022
影响因子：
--
通讯作者：
He, Yingchun
中科院分区：
文献类型：
--
作者：
Xiong, Yu;Zhong, Wenliang;Liu, Jie;Cheng, Bo;Fan, Jingying;Zhou, Fangliang;He, Lan;Tian, Daofa;He, Yingchun
为了揭示Luteolin的机制，叶黄素是传统中药（TCM）多边形的主要生物活性成分，可以抑制增殖并促进鼻咽癌（NPC）CNE2细胞中的凋亡。
基于中医系统的药理学数据库和分析平台（TCMSP），筛选了P. cuspidatum的生物活性化合物，筛选了潜在的靶基因和NPC疾病靶标的TCMSP，使用NPC的这些潜在靶标建立了关系网络，以及基因分析（GO）分析和基因的基因和基因的基因和基因的基因和基因群。使用体外实验（例如MTT，Cytation™5实时细胞监测，细胞周期检测，Annexin v-FITC/PI双染色，HOECHST 33342染色和线粒体膜电位（Δψm）检测，靶标和途径得到证实。
结果表明，发现了10种生物活性化合物（OB≥30％和DL≥0.18），发现与NPC相关的56个潜在的靶基因，这些靶基因包括Luteloin，quercetin和beta Sittosters，CASS cass cass，ccas，case cass，case case cass，case cass cass，case case cass，case casts cass，case case cass，case case cass，case cass，case，为pi3k-akt，jak/stat， MAPK和C型凝集素受体信号传导途径。与抗凋亡相关的蛋白质PCNA，抗凋亡蛋白XIAP和PI3K-AKT途径图相关的蛋白质P-ERK1/2，ERK1/2，AKT和PI3K，都改进了。
源自白斑假单胞菌的黄曲霉抑制了NPC CNE2细胞的增殖，并通过PI3K-AKT信号途径促进了细胞凋亡。
To reveal the mechanisms by which luteolin, the major bioactive component of the Traditional Chinese Medicine (TCM) Polygonum cuspidatum, inhibits proliferation and promotes apoptosis in nasopharyngeal carcinoma (NPC) CNE2 cells. Based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), bioactive compounds of P. cuspidatum, potential target genes and NPC disease targets of TCMSP were screened, relationship networks were constructed using these potential targets of NPC, and Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. The predicted compounds, targets and pathways were corroborated using in vitro experiments, such as MTT, Cytation™ 5 real-time cell monitoring, cell cycle detection, Annexin V-FITC/PI double staining, Hoechst 33342 staining, and mitochondrial membrane potential (ΔΨm) detection. The results showed that 10 bioactive compounds (OB ≥30% and DL ≥0.18), 157 potential target genes from P. cuspidatum, and 56 common targets related to NPC were found. These included important bioactive compounds such as luteolin, quercetin, and beta-sitosterol. Key common targets included EGFR, MYC, AKT1, CASP3, CCND1, ERBB2, and common targets were enriched for the PI3K-AKT, JAK/STAT, MAPK, and C-type lectin receptor signaling pathways. The binding energy of luteolin for six common targets was less than -5.0 kcal·mol−1. After luteolin (20 μM, and 40 μM) treatment to CNE2 cells for 36 h, cell survival rates decreased, accompanied by cell morphology changes, inhibition of the cell cycle at G2/M phase, and an induction of apoptosis. The expression of the cell proliferation related protein PCNA, the antiapoptosis protein XIAP, and the PI3K-AKT pathway diagram related proteins p-ERK1/2, ERK1/2, AKT, and PI3K, all decreased. Luteolin derived from P. cuspidatum inhibited the proliferation of NPC CNE2 cells and promoted cell apoptosis through the PI3K-AKT signal pathway.
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影响因子：
4
作者：
Chow, Shu-Er;Chen, Yu-Wen;Wang, Jong-Shyan
通讯作者：
Wang, Jong-Shyan
影响因子：
45.3
作者：
Hsu, Chiun;Lee, Se-Hoon;Hansen, Aaron R.
通讯作者：
Hansen, Aaron R.
影响因子：
6.1
作者：
Chen, Yaodong;Niu, Jiamei;Chen, Wu
通讯作者：
Chen, Wu
DOI：
10.1002/path.5130
发表时间：
2018-10
期刊：
The Journal of pathology
影响因子：
--
作者：
Lo AK;Lung RW;Dawson CW;Young LS;Ko CW;Yeung WW;Kang W;To KF;Lo KW
通讯作者：
Lo KW
DOI：
10.1002/hed.24341
发表时间：
2016-04-01
影响因子：
2.9
作者：
Lewis, Gary D.;Holliday, Emma B.;Frank, Steven J.
通讯作者：
Frank, Steven J.