The TIP30 protein complex, arachidonic acid and coenzyme A are required for vesicle membrane fusion.
The TIP30 protein complex, arachidonic acid and coenzyme A are required for vesicle membrane fusion.
复制标题
囊泡膜融合需要TIP30蛋白复合物，花生四烯酸和辅酶A。
DOI：
10.1371/journal.pone.0021233
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Xiao H
中科院分区：
文献类型：
--
作者：
Zhang C;Li A;Gao S;Zhang X;Xiao H
高效的膜融合已经在体外成功地利用人工膜和一些目前已知参与膜融合的细胞蛋白来模拟。然而，这些蛋白质不足以促进生物膜之间的有效融合，这表明关键的融合因子仍未确定。我们最近发现了一个含有TIP30、酰辅酶A合成酶长链家族成员4(ACSL4)和内嗜素B1(Endo B1)的TIP30蛋白复合体，它促进内吞小泡与Rab5a小泡的融合，在体内将内体酸化酶液泡(H+)-ATPase(V-ATPase)运输到早期内小体。在这里，我们证明了TIP30蛋白复合体在体外促进内吞小泡与Rab5a小泡的融合。这两个囊泡的融合还依赖于花生四烯酸、辅酶A和ACSL4合成花生四烯基辅酶A。此外，TIP30复合体能够将花生四烯基团转移到磷脂酸(PA)上，产生一种新的脂类物种，能够诱导膜之间的紧密接触。综上所述，我们的数据表明，TIP30复合体通过修饰膜上的PA来促进生物膜融合。
Efficient membrane fusion has been successfully mimicked in vitro using artificial membranes and a number of cellular proteins that are currently known to participate in membrane fusion. However, these proteins are not sufficient to promote efficient fusion between biological membranes, indicating that critical fusogenic factors remain unidentified. We have recently identified a TIP30 protein complex containing TIP30, acyl-CoA synthetase long-chain family member 4 (ACSL4) and Endophilin B1 (Endo B1) that promotes the fusion of endocytic vesicles with Rab5a vesicles, which transport endosomal acidification enzymes vacuolar (H+)-ATPases (V-ATPases) to the early endosomes in vivo. Here, we demonstrate that the TIP30 protein complex facilitates the fusion of endocytic vesicles with Rab5a vesicles in vitro. Fusion of the two vesicles also depends on arachidonic acid, coenzyme A and the synthesis of arachidonyl-CoA by ACSL4. Moreover, the TIP30 complex is able to transfer arachidonyl groups onto phosphatidic acid (PA), producing a new lipid species that is capable of inducing close contact between membranes. Together, our data suggest that the TIP30 complex facilitates biological membrane fusion through modification of PA on membranes.
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