Assessment of mass transfer and mixing in rigid lab‐scale disposable bioreactors at low power input levels
Assessment of mass transfer and mixing in rigid lab‐scale disposable bioreactors at low power input levels
复制标题
低功率输入水平下刚性实验室规模一次性生物反应器中的传质和混合评估
DOI：
10.1002/btpr.1981
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发表时间：
2014
影响因子：
2.9
通讯作者：
W. Bakker
中科院分区：
文献类型：
--
作者：
G. van Eikenhorst;Y. Thomassen;L. A. van der Pol;W. Bakker
在刚性一次性搅拌罐生物反应器中测量传质、混合时间和功耗，并与传统玻璃生物反应器的传质、混合时间和功耗进行比较。体积传质系数和混合时间通常在高搅拌速度下结合用于单细胞悬浮液和大多数细菌培养物的鼓泡曝气来确定。相比之下，在此应用低搅拌速度与顶空通气的组合。这些设置通常用于培养粘附于微载体生长的哺乳动物细胞。刚性一次性容器显示出与传统玻璃生物反应器相似的工程特性。基于所呈现的结果，可以选择贴壁细胞培养的适当设置，通常在5 W m-3的最大功率输入水平下操作。根据所使用的一次性生物反应器，搅拌器速度范围为38至147 rpm将导致5 W m-3的功率输入。该功率输入将在22 ± 1 s内将流体混合到95%的程度，并产生0.46 ± 0.07 h−1的体积传质系数。© 2014美国化学工程师学会生物技术。程序：30：1269-1276，2014
Mass transfer, mixing times and power consumption were measured in rigid disposable stirred tank bioreactors and compared to those of a traditional glass bioreactor. The volumetric mass transfer coefficient and mixing times are usually determined at high agitation speeds in combination with sparged aeration as used for single cell suspension and most bacterial cultures. In contrast, here low agitation speeds combined with headspace aeration were applied. These settings are generally used for cultivation of mammalian cells growing adherent to microcarriers. The rigid disposable vessels showed similar engineering characteristics compared to a traditional glass bioreactor. On the basis of the presented results appropriate settings for adherent cell culture, normally operated at a maximum power input level of 5 W m−3, can be selected. Depending on the disposable bioreactor used, a stirrer speed ranging from 38 to 147 rpm will result in such a power input of 5 W m−3. This power input will mix the fluid to a degree of 95% in 22 ± 1 s and produce a volumetric mass transfer coefficient of 0.46 ± 0.07 h−1. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:1269–1276, 2014
影响因子：
4
作者：
A. Oller;C. Buser;M. Tyo;W. Thilly
通讯作者：
A. Oller;C. Buser;M. Tyo;W. Thilly