Acetate and succinate benefit host muscle energetics as exercise-associated post-biotics.
Acetate and succinate benefit host muscle energetics as exercise-associated post-biotics.
复制标题
DOI：
10.14814/phy2.15848
复制
发表时间：
2023-11
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
--
作者：
最近，肠道微生物群已成为运动诱导的系统适应的有效调节剂，并且似乎对调节运动的一些益处至关重要。这项研究建立在先前的证据基础上，建立了肠道微生物群-骨骼肌轴，确定了运动引起的微生物群组成的变化。对非运动训练对照组和运动训练小鼠的粪便样本进行宏基因组测序。生物多样性指数表明，运动训练没有改变α多样性。然而，在训练和未训练的微生物组之间，β‐多样性存在显著差异。运动显著提高了Muribaculaceae细菌DSM 103720的水平。细菌生长的计算模拟被用来预测运动反应性细菌在硅培养下积累的代谢物。我们确定醋酸盐和琥珀酸盐是Muribaculaceae细菌产生的潜在肠道微生物代谢物，然后在机械过载诱导的肌肉肥大期间给小鼠服用。虽然在机械过载引起的肥厚的前5天内，琥珀酸盐和琥珀酸盐对整体肌肉生长的反应没有观察到差异，但乙酸盐和琥珀酸盐增加了骨骼肌线粒体呼吸。当作为后生物制剂给予时，琥珀酸盐或醋酸盐治疗可改善肌肉肥大期间的氧化代谢。对渐进式加权轮跑（PoWeR）后的粪便DNA进行宏基因组测序，发现Muribaculaceae细菌的丰度增加。细菌生长的计算模拟预测醋酸盐和琥珀酸盐是这些细菌产生的代谢物，醋酸盐和琥珀酸盐在机械过载（MOV） 5天后增加了线粒体呼吸。
Recently, the gut microbiome has emerged as a potent modulator of exercise‐induced systemic adaptation and appears to be crucial for mediating some of the benefits of exercise. This study builds upon previous evidence establishing a gut microbiome‐skeletal muscle axis, identifying exercise‐induced changes in microbiome composition. Metagenomics sequencing of fecal samples from non‐exercise‐trained controls or exercise‐trained mice was conducted. Biodiversity indices indicated exercise training did not change alpha diversity. However, there were notable differences in beta‐diversity between trained and untrained microbiomes. Exercise significantly increased the level of the bacterial species Muribaculaceae bacterium DSM 103720. Computation simulation of bacterial growth was used to predict metabolites that accumulate under in silico culture of exercise‐responsive bacteria. We identified acetate and succinate as potential gut microbial metabolites that are produced by Muribaculaceae bacterium, which were then administered to mice during a period of mechanical overload‐induced muscle hypertrophy. Although no differences were observed for the overall muscle growth response to succinate or acetate administration during the first 5 days of mechanical overload‐induced hypertrophy, acetate and succinate increased skeletal muscle mitochondrial respiration. When given as post‐biotics, succinate or acetate treatment may improve oxidative metabolism during muscle hypertrophy. Metagenomic sequencing of fecal DNA after progressive weighted wheel running (PoWeR) was performed, identifying increased abundance of Muribaculaceae bacterium. Computational simulation of bacterial growth predicted acetate and succinate as metabolites produced by these bacteria, and administration of acetate and succinate increased mitochondrial respiration after 5 days of mechanical overload (MOV).
登录
查看更多内容
影响因子：
5.9
作者：
Diener C;Hoge ACH;Kearney SM;Kusebauch U;Patwardhan S;Moritz RL;Erdman SE;Gibbons SM
通讯作者：
Gibbons SM
影响因子：
4.8
作者：
Carey RA;Montag D
通讯作者：
Montag D
影响因子：
7.7
作者：
Beghini F;McIver LJ;Blanco-Míguez A;Dubois L;Asnicar F;Maharjan S;Mailyan A;Manghi P;Scholz M;Thomas AM;Valles-Colomer M;Weingart G;Zhang Y;Zolfo M;Huttenhower C;Franzosa EA;Segata N
通讯作者：
Segata N
影响因子：
6
作者：
Bakare AB;Rao RR;Iyer S
通讯作者：
Iyer S
DOI：
10.1093/function/zqac027
发表时间：
2022
期刊：
Function (Oxford, England)
影响因子：
--
作者：
通讯作者：
--