The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability.
The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability.
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DOI：
10.1371/journal.pone.0037434
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Rodrigues-Pousada C
中科院分区：
文献类型：
--
作者：
Pimentel C;Vicente C;Menezes RA;Caetano S;Carreto L;Rodrigues-Pousada C
芽殖酵母酿酒酵母（Saccharomyces cerevisiae）已经开发出几种机制来避免缺铁的严重后果或铁过量的毒性作用。在这项工作中，我们分析了全球基因表达的变化发生在酵母细胞经历铁超载。直接或间接参与铁稳态的几个基因表现出改变表达和这些变化的相关性进行了讨论。还进行了微阵列分析以鉴定铁响应因子Yap5的新靶点。除了铁液泡转运蛋白CCC1，Yap5还控制谷氧还蛋白GRX4的表达，先前已知GRX4参与调节Aft1核定位。一致地，我们表明，在没有Yap5 Aft1的核排斥是轻微受损。这些研究提供了进一步的证据，表明细胞通过多种途径控制铁稳态。
The budding yeast Saccharomyces cerevisiae has developed several mechanisms to avoid either the drastic consequences of iron deprivation or the toxic effects of iron excess. In this work, we analysed the global gene expression changes occurring in yeast cells undergoing iron overload. Several genes directly or indirectly involved in iron homeostasis showed altered expression and the relevance of these changes are discussed. Microarray analyses were also performed to identify new targets of the iron responsive factor Yap5. Besides the iron vacuolar transporter CCC1, Yap5 also controls the expression of glutaredoxin GRX4, previously known to be involved in the regulation of Aft1 nuclear localization. Consistently, we show that in the absence of Yap5 Aft1 nuclear exclusion is slightly impaired. These studies provide further evidence that cells control iron homeostasis by using multiple pathways.
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