Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes.
Apolipoprotein M mediates sphingosine-1-phosphate efflux from erythrocytes.
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DOI：
10.1038/s41598-017-15043-y
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发表时间：
2017-11-08
影响因子：
4.6
通讯作者：
Christoffersen C
中科院分区：
文献类型：
--
作者：
Christensen PM;Bosteen MH;Hajny S;Nielsen LB;Christoffersen C
鞘氨醇-1-磷酸(S1P)是一种生物活性脂质，与血管生成、淋巴细胞转运和内皮屏障功能有关。红细胞、血小板和血管内皮细胞是血浆S1P的主要来源。高密度脂蛋白中载脂蛋白M(ApoM)携带70%的血浆S1P，而30%由白蛋白携带。目前的目的是研究载脂蛋白在人红细胞S1P输出中的作用。红细胞比白蛋白更有效地将S1P输出到高密度脂蛋白，特别是当载脂蛋白存在于高密度脂蛋白中时。相比之下，鞘氨醇向高密度脂蛋白的出口不受载脂蛋白的存在的影响。载脂蛋白促进S1P输出的特异性不依赖于载脂蛋白与高密度脂蛋白颗粒的结合。用ABCC1转运蛋白的抑制剂MK-571处理后，S1P从人红细胞到载脂蛋白的出口有效减少，而ABCB1或ATPase的抑制剂不影响S1P的出口。因此，ABCC1可能参与了S1P从红细胞到载脂蛋白的输出。
Sphingosine-1-phosphate (S1P) is a bioactive lipid implicated in e.g. angiogenesis, lymphocyte trafficking, and endothelial barrier function. Erythrocytes are a main source of plasma S1P together with platelets and endothelial cells. Apolipoprotein M (apoM) in HDL carries 70% of plasma S1P, whereas 30% is carried by albumin. The current aim was to investigate the role of apoM in export of S1P from human erythrocytes. Erythrocytes exported S1P more efficiently to HDL than to albumin, particularly when apoM was present in HDL. In contrast, export of sphingosine to HDL was unaffected by the presence of apoM. The specific ability of apoM to promote export of S1P was independent of apoM being bound in HDL particles. Treatment with MK-571, an inhibitor of the ABCC1 transporter, effectively reduced export of S1P from human erythrocytes to apoM, whereas the export was unaffected by inhibitors of ABCB1 or ATPase. Thus, ABCC1 could be involved in export of S1P from erythrocytes to apoM.
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