The fucosyltransferase FucT-VII regulates E-selectin ligand synthesis in human T cells.
The fucosyltransferase FucT-VII regulates E-selectin ligand synthesis in human T cells.
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岩藻糖基转移酶FUCT-VII调节人T细胞中的E-选择蛋白配体合成。
DOI：
10.1083/jcb.133.4.911
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发表时间：
1996-05
影响因子：
7.8
通讯作者：
Stoolman, LM
中科院分区：
文献类型：
--
作者：
Knibbs, RN;Craig, RA;Natsuka, S;Chang, A;Cameron, M;Lowe, JB;Stoolman, LM
T细胞上的选择配体有助于循环细胞进入皮肤和其他地方的慢性炎性病变。该报告提供了第一个证据，证明一个单一的聚焦转移酶，称为FucT-VII，控制人类t淋巴细胞中E-选择素配体的合成。相比之下，fft - iv转移酶（ELFT酶）构建的e -选择素配体的亲和力较低，并且只需要在髓细胞中发现的酶水平。用肉豆蔻酸酯phorbol处理Jurkat细胞增加了唾液化Lewis(x)相关sLe(x)相关表位的表达，并诱导了e -选择素配体的合成，这些配体在线性剪切应力的生理水平上具有功能。Northern分析显示稳态水平的fft - vii mRNA平行增加，但其他两种白细胞相关的聚焦转移酶（fft - iv和VI）没有增加。在Jurkat细胞中稳定转染FucT-VII基因诱导了高水平的sLe(x)相关表位和e -选择素配体的合成，其速度等于或超过循环淋巴细胞上的配体。在诱导sLe（x,a）表位表达的条件下，t淋巴细胞的生长增加了FucT-VII mRNA的水平，通过无细胞提取物合成了唾液化lewis (x)结构，并合成了e -选择素配体，其数量与在FucT-VII上的配体相当。相反，在t淋巴细胞中，与e -选择素配体合成相关的fft - iv和VI酶的mRNA水平和活性均未增加。髓系细胞与淋巴母细胞不同，在结合e -选择素配体时表达高水平的FucT- VII和IV酶，这提高了这两种酶都参与配体合成的可能性。FucT-IV转染的Jurkat细胞合成了e -选择素的低亲和力配体，但仅与CDw65 （VIM-2）碳水化合物表位相关。只有血液中性粒细胞和骨髓细胞系在与e配体合成相关的水平上表达该表位。相比之下，天然Jurkat细胞、血液单核细胞、血液淋巴细胞和培养的t淋巴细胞表达低水平或不表达。我们得出结论，FucT-VII是人t淋巴细胞e -选择素配体合成的主要调节剂，而FucT-VII和FucT-IV可能指导某些髓细胞的配体合成。
Selectin-ligands on T cells contribute to the recruitment of circulating cells into chronic inflammatory lesions in the skin and elsewhere. This report provides the first evidence that a single fucosyltransferase, termed FucT-VII, controls the synthesis of E- selectin ligands in human T-lymphoblasts. The FucT-IV transferase (the ELFT enzyme), in contrast constructs lower avidity E-selectin ligands and requires enzyme levels found only in myeloid cells. Treatment of Jurkat cells with phorbol myristate acetate increased the expression of sialylated Lewis(x)-related sLe(x)related epitopes and induced the synthesis of E-selectin ligands functional at physiologic levels of linear shear-stress. Northern analysis revealed a parallel increase in the steady-state levels FucT-VII mRNA, but there were no increases in the two other leukocyte-associated fucosyltransferases (FucT-IV and VI). The stable transfection of the FucT-VII gene into Jurkat cells induced high levels of the sLe(x)-related epitopes and the synthesis of E-selectin ligands which equal or exceeded the avidity of those on circulating lymphocytes. The growth of T-lymphoblasts under conditions which induced expression of the sLe(x,a) epitopes increased the level of FucT-VII mRNA, the synthesis of sialylated-Lewis(x) structures by cell-free extracts and the synthesis of E-selectin ligands equal in avidity to those on FucT-VII transfectants. In contrast, neither the mRNA levels nor activities of the FucT-IV and VI enzymes increased in association with E-selectin ligand synthesis in T-lymphoblasts. Myeloid cell lines, unlike lymphoblasts, expressed high levels of both the FucT- VII and IV enzymes in conjunction with E-selectin ligands raising the possibility that both enzymes contributed to ligand synthesis. FucT-IV transfected Jurkat cells synthesized low avidity ligands for E-selectin but only in association with CDw65 (VIM-2) carbohydrate epitope. Only blood neutrophils and myeloid cell lines expressed this epitope at the levels associated with E-ligand synthesis in the transfectants. In contrast, native Jurkat cells, blood monocytes, blood lymphocytes, and cultured T-lymphoblasts expressed low levels or none. We conclude that FucT-VII is a principal regulator of E-selectin ligand synthesis in human T-lymphoblasts while both FucT-VII and FucT-IV may direct ligand synthesis in some myeloid cells.
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影响因子：
56.9
作者：
PHILLIPS, ML;NUDELMAN, E;PAULSON, JC
通讯作者：
PAULSON, JC
影响因子：
4.8
作者：
GERSTEN, KM;NATSUKA, S;LOWE, JB
通讯作者：
LOWE, JB
DOI：
10.1084/jem.174.6.1461
发表时间：
1991-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Berg EL;Yoshino T;Rott LS;Robinson MK;Warnock RA;Kishimoto TK;Picker LJ;Butcher EC
通讯作者：
Butcher EC
影响因子：
15.9
作者：
ABBASSI, O;KISHIMOTO, TK;SMITH, CW
通讯作者：
SMITH, CW
影响因子：
15.9
作者：
GROBER, JS;BOWEN, BL;STOOLMAN, LM
通讯作者：
STOOLMAN, LM