miR-20b is up-regulated in brain metastases from primary breast cancers.
miR-20b is up-regulated in brain metastases from primary breast cancers.
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DOI：
10.18632/oncotarget.3664
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发表时间：
2015-05-20
期刊：
影响因子：
--
通讯作者：
Sarkar FH
中科院分区：
文献类型：
--
作者：
Ahmad A;Ginnebaugh KR;Sethi S;Chen W;Ali R;Mittal S;Sarkar FH
脑转移在晚期乳腺癌患者中很常见，并且与预后不良相关。然而，尚未建立独特的分子生物标志物。我们假设microRNA-20 b（miR-20 b）在乳腺癌脑转移中起作用。我们的研究队列包括11例伴有脑转移的乳腺癌患者和9例对照患者（年龄、分期和随访匹配），这些患者患有乳腺癌但不伴有脑转移。对病例进行显微镜检查以选择具有>50%肿瘤细胞的肿瘤块，从福尔马林固定的石蜡包埋（FFPE）肿瘤组织块中提取RNA，并使用qRT-PCR分析miR-20 b的表达。我们使用MCF-7和MDA-MB-231细胞进一步测试了miR-20 b过表达对体外集落形成和侵袭的影响。在患者来源的样本中，与原发性乳腺肿瘤以及没有脑转移的患者相比，乳腺癌患者脑转移中的miR-20 b表达显著更高。miR-20 b还显著诱导乳腺癌细胞的殖民地形成和侵袭性。此外，与骨转移细胞相比，在脑转移细胞中观察到miR-20 b水平较高。总之，我们的研究结果表明miR-20 b在乳腺癌脑转移中的新作用，值得进一步研究其作为预后和/或治疗靶点的潜力。
Brain metastases are frequent in patients with advanced breast cancer and are associated with poor prognosis. However, unique molecular biomarkers have not yet been established. We hypothesized that microRNA-20b (miR-20b) plays a role in breast cancer brain metastasis. Our study cohort comprised of eleven breast cancer patients with brain metastasis and nine control patients (age, stage, and follow-up matched) with breast cancer without brain metastasis. Cases were reviewed microscopically to select tumor blocks with >50% tumor cells, RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue blocks and expression of miR-20b analyzed using qRT-PCR. We further tested the effect of miR-20b overexpression on colony formation and invasion in vitro using MCF-7 and MDA-MB-231 cells. In the patient-derived samples, miR-20b expression was significantly higher in brain metastases of breast cancer patients, compared to primary breast tumors as well as the patients without brain metastasis. miR-20b also significantly induced the colony formation and invasiveness of breast cancer cells. Further, miR-20b levels were observed to be high in brain-metastasizing cells, compared to bone-metastasizing cells. Together, our findings suggest a novel role of miR-20b in breast cancer brain metastasis that warrants further investigation for its potential to be developed as prognostic and/or therapeutic target.
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早期生长反应1在miR-20b转录调节乳腺癌中的关键作用。
影响因子：
--
作者：
Li D;Ilnytskyy Y;Kovalchuk A;Khachigian LM;Bronson RT;Wang B;Kovalchuk O
通讯作者：
Kovalchuk O
DOI：
10.1111/j.1440-1746.2008.05666.x
发表时间：
2009-04-01
影响因子：
4.1
作者：
Guo, Junming;Miao, Ying;Wang, Yanjun
通讯作者：
Wang, Yanjun
影响因子：
11.5
作者：
Hui, Angela B. Y.;Lin, Alice;Liu, Fei-Fei
通讯作者：
Liu, Fei-Fei
DOI：
10.1073/pnas.0906947106
发表时间：
2009-09-15
影响因子：
11.1
作者：
Castellano, Leandro;Giamas, Georgios;Stebbing, Justin
通讯作者：
Stebbing, Justin
microRNA在乳腺癌迁移，侵袭和转移中的作用。
影响因子：
5.6
作者：
Tang J;Ahmad A;Sarkar FH
通讯作者：
Sarkar FH