Loss of alternately spliced messenger RNA of the luteinizing hormone receptor and stability of the follicle-stimulating hormone receptor messenger RNA in granulosa cell tumors of the human ovary.
Loss of alternately spliced messenger RNA of the luteinizing hormone receptor and stability of the follicle-stimulating hormone receptor messenger RNA in granulosa cell tumors of the human ovary.
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人卵巢颗粒细胞瘤中黄体生成素受体交替剪接信使 RNA 的丢失和促卵泡激素受体信使 RNA 的稳定性。
DOI：
10.1006/gyno.2000.5946
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Roche,PC
中科院分区：
文献类型：
--
作者：
Reinholz,MM;Zschunke,MA;Roche,PC
目的检测正常人黄体和颗粒细胞瘤中LH受体（LHR）和FSH受体（FSHR）mRNA的表达，方法采用RT/PCR和DNA测序技术检测正常人黄体和颗粒细胞瘤中LH受体mRNA的表达。同种型1的外显子II和III部分缺失，同种型2的外显子IX缺失。同种型3还具有外显子II和III的部分缺失以及外显子IX的全部缺失。外显子III至VI在同种型4-7中缺失。同种型5也缺失外显子IX，同种型6也缺失部分外显子XI。亚型7也有外显子IX和部分外显子XI缺失。在FIGO I-IV期颗粒细胞瘤中观察到LHR mRNA亚型的异常迁移模式。III-IV期的5个肿瘤样品中不存在许多同种型。7个I期样本具有取决于肿瘤大小的异常迁移模式。随着肿瘤大小的增加，LHR mRNA亚型的异常迁移模式更加明显，并且未检测到某些亚型。FL和至少一个额外的亚型被确定为FSHR mRNA。亚型1的外显子IV和V区域缺失。FSHR mRNA亚型有一个类似的迁移模式为正常卵巢和颗粒细胞tumors. ConclusionsAlternatively剪接的LHR和FSHR的mRNA的形式存在正常人卵巢和颗粒细胞肿瘤。颗粒细胞瘤中的异常迁移和缺失LHR mRNA亚型似乎不是由与肿瘤进展相关的一般基因组不稳定性引起的。这些发现对于理解选择性剪接在不同病理状态下LHR和FSHR表达调控中的作用具有重要意义。
ObjectivesThe expression of messenger RNA (mRNA) for the LH (LHR) and FSH receptors (FSHR) was examined in normal human corpora lutea and granulosa cell tumors.MethodsExpression was examined by RT/PCR and DNA sequencing techniques.ResultsThe full-length (FL) coding region and seven additional isoforms were identified for normal LHR mRNA. Isoform 1 had portions of exons II and III deleted, and isoform 2 had exon IX omitted. Isoform 3 also had portions of exons II and III deleted and all of exon IX deleted. Exons III through VI were missing in isoforms 4–7. Isoform 5 also had exon IX omitted, and isoform 6 also had part of exon XI missing. Isoform 7 also had exon IX and part of exon XI deleted. An aberrant migration pattern of the LHR mRNA isoforms was observed for granulosa cell tumors with FIGO Stage I–IV. Five tumor samples of Stage III–IV had many isoforms absent. Seven Stage I samples had aberrant migration patterns that depended on the size of the tumor. As the size of the tumor increased the aberrant migration pattern of the LHR mRNA isoforms was more pronounced and some isoforms were not detected. The FL and at least one additional isoform were identified for FSHR mRNA. Isoform 1 had regions of exons IV and V deleted. The FSHR mRNA isoforms had a similar migration pattern for the normal ovary and the granulosa cell tumors.ConclusionsAlternately spliced forms of mRNA for the LHR and FSHR exist for normal human ovary and granulosa cell tumors. The aberrant migration and missing LHR mRNA isoforms in granulosa cell tumors do not appear to result from general genomic instability associated with tumor progression. These findings are important to understand the role of alternate splicing in the regulation of LHR and FSHR expression in different pathological states.
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影响因子：
20.3
作者：
J. Richards
通讯作者：
J. Richards
影响因子：
158.5
作者：
ROSSING, MA;DALING, JR;SELF, SG
通讯作者：
SELF, SG
DOI：
10.1016/s0021-9258(18)42858-x
发表时间：
1992-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Thomas Gudermann;Mariel Birnbaumer;Lutz Birnbaumer
通讯作者：
Thomas Gudermann;Mariel Birnbaumer;Lutz Birnbaumer
DOI：
10.1016/0020-7292(94)90060-4
发表时间：
1993
期刊：
The Lancet
影响因子：
--
作者：
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通讯作者：
B. Garadnay
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影响因子：
3.8
作者：
R. Nakano;S. Kitayama;M. Yamoto;K. Shima;Akira Ooshima
通讯作者：
Akira Ooshima