Evidence for niche adaptation in the genome of the bovine pathogen Streptococcus uberis.
Evidence for niche adaptation in the genome of the bovine pathogen Streptococcus uberis.
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DOI：
10.1186/1471-2164-10-54
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发表时间：
2009-01-28
期刊：
影响因子：
4.4
通讯作者：
Parkhill J
中科院分区：
文献类型：
--
作者：
Ward PN;Holden MT;Leigh JA;Lennard N;Bignell A;Barron A;Clark L;Quail MA;Woodward J;Barrell BG;Egan SA;Field TR;Maskell D;Kehoe M;Dowson CG;Chanter N;Whatmore AM;Bentley SD;Parkhill J
uberis链球菌是一种革兰氏阳性细菌病原体，在商业奶牛群中造成很大比例的牛乳腺炎，它在奶牛的多个身体部位定植，包括肠道、生殖道和乳腺。对牛链球菌0140J的全基因组序列进行了比较分析，以帮助阐明这种有效的牛病原体的生物学特性。基因组共发现1825个预测编码序列（CDSs），其中62个被鉴定为假基因或基因片段。与相关的化脓性链球菌比较，鉴定出同源cds的保守核心（40%）。有趣的是，与其他化脓性链球菌相比，S. uberis 0140J显示出较少的可移动遗传元件，然而噬菌体衍生岛和假定的基因组岛被确定。比较基因组学分析显示，无乳链球菌和马链球菌的基因组最相似。zooepidemicus。相比之下，11%的CDSs未鉴定出链球菌同源物，这表明要么是祖先序列的独特保留，要么是从其他来源获得序列。功能包括运输、分解代谢、调节和编码细胞包膜蛋白的CDSs在这个独特的基因集中被过度代表；鉴定出一系列有限的假定毒性cds。S. uberis利用来自多种代谢选择的营养灵活性，成功地占据了一个离散的生态位。在uberis中观察到的特征强烈暗示了一种适应挑战性和不断变化的环境参数的机会性病原体。
Streptococcus uberis, a Gram positive bacterial pathogen responsible for a significant proportion of bovine mastitis in commercial dairy herds, colonises multiple body sites of the cow including the gut, genital tract and mammary gland. Comparative analysis of the complete genome sequence of S. uberis strain 0140J was undertaken to help elucidate the biology of this effective bovine pathogen. The genome revealed 1,825 predicted coding sequences (CDSs) of which 62 were identified as pseudogenes or gene fragments. Comparisons with related pyogenic streptococci identified a conserved core (40%) of orthologous CDSs. Intriguingly, S. uberis 0140J displayed a lower number of mobile genetic elements when compared with other pyogenic streptococci, however bacteriophage-derived islands and a putative genomic island were identified. Comparative genomics analysis revealed most similarity to the genomes of Streptococcus agalactiae and Streptococcus equi subsp. zooepidemicus. In contrast, streptococcal orthologs were not identified for 11% of the CDSs, indicating either unique retention of ancestral sequence, or acquisition of sequence from alternative sources. Functions including transport, catabolism, regulation and CDSs encoding cell envelope proteins were over-represented in this unique gene set; a limited array of putative virulence CDSs were identified. S. uberis utilises nutritional flexibility derived from a diversity of metabolic options to successfully occupy a discrete ecological niche. The features observed in S. uberis are strongly suggestive of an opportunistic pathogen adapted to challenging and changing environmental parameters.
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影响因子：
3.7
作者：
Beres SB;Musser JM
通讯作者：
Musser JM
DOI：
10.1016/j.ijfoodmicro.2007.08.014
发表时间：
2008-09-01
影响因子：
5.4
作者：
Delorme, Christine
通讯作者：
Delorme, Christine
影响因子：
3.2
作者：
Candon, Heather L.;Allan, Brenda J.;Gaynor, Erin C.
通讯作者：
Gaynor, Erin C.
影响因子：
2.1
作者：
BRAMLEY, AJ
通讯作者：
BRAMLEY, AJ
影响因子：
3.5
作者：
Compton, C. W. R.;Heuer, C.;McDougall, S.
通讯作者：
McDougall, S.