Prevalence and genetic profiling of virulence determinants of non-O157 Shiga toxin-producing Escherichia coli isolated from cattle, beef, and humans, Calcutta, India.
Prevalence and genetic profiling of virulence determinants of non-O157 Shiga toxin-producing Escherichia coli isolated from cattle, beef, and humans, Calcutta, India.
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印度加尔各答，从牛、牛肉和人类中分离出的非 O157 志贺毒素大肠杆菌的流行率和毒力决定因素的基因分析。
DOI：
10.3201/eid0801.010104
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发表时间：
2002
影响因子：
11.8
通讯作者：
G. Nair
中科院分区：
文献类型：
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作者：
A. Khan;S. Yamasaki;T. Sato;T. Ramamurthy;A. Pal;S. Datta;N. Chowdhury;S. Das;A. Sikdar;T. Tsukamoto;S. Bhattacharya;Y. Takeda;G. Nair
我们调查了产滋贺毒素大肠杆菌（STEC）在印度加尔各答住院腹泻患者中的流行情况，以及从该市屠宰场收集的健康家畜和生牛肉样本。多重聚合酶链反应，使用特异性引物的stx 1和stx 2检测STEC在18%的奶牛粪便样本，50%的生牛肉样本，1.4%和0.6%的血便和水样便样本，分别从住院腹泻患者。在STEC分离株的各种毒力基因表明，stx 1等位基因占主导地位。还鉴定了质粒携带的标记物，即hlyA、katP、espP和etpD。采用磁珠酶联免疫吸附试验和Vero细胞试验检测和评价菌株产生的滋贺毒素的细胞毒作用。STEC不是印度腹泻的重要原因;然而，其在国内牛和牛肉样品中的存在表明，这种肠道病原体可能成为未来的主要公共卫生问题。
We investigated the prevalence of Shiga toxin-producing Escherichia coli (STEC) in hospitalized diarrhea patients in Calcutta, India, as well as in healthy domestic cattle and raw beef samples collected from the city's abattoir. Multiplex polymerase chain reaction using primers specific for stx1 and stx2 detected STEC in 18% of cow stool samples, 50% of raw beef samples, and 1.4% and 0.6% of bloody and watery stool samples, respectively, from hospitalized diarrhea patients. Various virulence genes in the STEC isolates indicated that stx1 allele predominated. Plasmid-borne markers, namely, hlyA, katP, espP, and etpD, were also identified. Bead enzyme-linked immunosorbent assay and Vero cell assay were performed to detect and evaluate the cytotoxic effect of the Shiga toxins produced by the strains. STEC is not an important cause of diarrhea in India; however, its presence in domestic cattle and beef samples suggests that this enteropathogen may become a major public health problem in the future.
通过 DNA 菌落杂交检测产肠毒素大肠杆菌。
DOI：
10.1093/infdis/142.6.892
发表时间：
1980
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Moseley,SL;Huq,I;Alim,AR;So,M;Samadpour-Motalebi,M;Falkow,S
通讯作者：
Falkow,S