Comparative transcriptional analysis of Capsicum flower buds between a sterile flower pool and a restorer flower pool provides insight into the regulation of fertility restoration
Comparative transcriptional analysis of Capsicum flower buds between a sterile flower pool and a restorer flower pool provides insight into the regulation of fertility restoration
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对不育花池和恢复花池之间的辣椒花芽进行比较转录分析，有助于深入了解生育力恢复的调节
DOI：
10.1186/s12864-019-6210-3
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发表时间：
2019-11
期刊：
影响因子：
4.4
通讯作者：
Ru Zhang
中科院分区：
文献类型：
--
作者：
Bingqiang Wei;Lanlan Wang;Paul Bosl;Gaoyuan Zhang;Ru Zhang
研究背景细胞质雄性不育(CMS)及其育性恢复(Rf)系统是产生F1杂种种子的重要机制。了解在分子水平上控制恢复的相互作用将使植物育种者受益。 CMS 是由线粒体和核基因之间的相互作用引起的，CMS 表型无法产生功能性花药、花粉或雄配子。因此，了解花药和花粉发育的复杂过程是了解 CMS 系统的先决条件。目前普遍认为细胞核中的Rf基因可恢复CMS的育性，但Rf基因尚未被克隆。在本研究中，CMS系8A和Rf系R1以及不育库（SP）的种质和恢复系库（RP）利用RNA测序和批量分离分析相结合的方法分析了CMS及其育性恢复系之间的差异表达基因（DEG）。结果与8A相比，R1中总共有2274个基因上调，而R1中共有1490个基因上调。 与 SP 相比，RP 中的基因上调。与不育种质8A和SP相比，两种恢复系种质R1和RP中有891个基因上调。通过对共上调表达基因的注释和表达分析，筛选出8个与生育力恢复相关的基因。这些基因编码假定的果糖激酶、磷脂酰肌醇4-磷酸5-激酶、果胶酸裂合酶、外聚半乳糖醛酸酶、果胶酯酶、纤维素合酶、成束蛋白样阿拉伯半乳聚糖蛋白和磷酸肌醇磷脂酶C。此外，磷脂酰肌醇信号传导系统和肌醇磷酸代谢相关 结论我们的研究揭示了8个基因与育性恢复相关，这为理解辣椒CMS育性恢复的分子机制提供了新的见解。
BackgroundCytoplasmic male sterility (CMS) and its restoration of fertility (Rf) system is an important mechanism to produce F1hybrid seeds. Understanding the interaction that controls restoration at a molecular level will benefit plant breeders. The CMS is caused by the interaction between mitochondrial and nuclear genes, with the CMS phenotype failing to produce functional anthers, pollen, or male gametes. Thus, understanding the complex processes of anther and pollen development is a prerequisite for understanding the CMS system. Currently it is accepted that theRfgene in the nucleus restores the fertility of CMS, however theRfgene has not been cloned. In this study, CMS line 8A and the Rf line R1, as well as a sterile pool (SP) of accessions and a restorer pool (RP) of accessions analyzed the differentially expressed genes (DEGs) between CMS and its fertility restorer using the conjunction of RNA sequencing and bulk segregation analysis.ResultsA total of 2274 genes were up-regulated in R1 as compared to 8A, and 1490 genes were up-regulated in RP as compared to SP. There were 891 genes up-regulated in both restorer accessions, R1 and RP, as compared to both sterile accessions, 8A and SP. Through annotation and expression analysis of co-up-regulated expressed genes, eight genes related to fertility restoration were selected. These genes encode putative fructokinase, phosphatidylinositol 4-phosphate 5-kinase, pectate lyase, exopolygalacturonase, pectinesterase, cellulose synthase, fasciclin-like arabinogalactan protein and phosphoinositide phospholipase C. In addition, a phosphatidylinositol signaling system and an inositol phosphate metabolism related to the fertility restorer of CMS were ranked as the most likely pathway for affecting the restoration of fertility in pepper.ConclusionsOur study revealed that eight genes were related to the restoration of fertility, which provides new insight into understanding the molecular mechanism of fertility restoration of CMS inCapsicum.
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影响因子：
3.1
作者：
Dieterich, JH;Braun, HP;Schmitz, UK
通讯作者：
Schmitz, UK
影响因子：
5.3
作者：
通讯作者：
--
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
7.8
作者：
Kost, B;Lemichez, E;Spielhofer, P;Hong, Y;Tolias, K;Carpenter, C;Chua, N H
通讯作者：
Chua, N H
五肽重复位点的插入删除与萝卜（Raphanus sativus L.）的育性转变为细胞质雄性不育有关
影响因子：
3.1
作者：
Zhiwei Wang;Chu;e Wang;Shiyong Mei;Lei gao;Yuan Zhou;Ting Wang
通讯作者：
Ting Wang