Comparative Analysis of Fruit Ripening-Related miRNAs and Their Targets in Blueberry Using Small RNA and Degradome Sequencing.
Comparative Analysis of Fruit Ripening-Related miRNAs and Their Targets in Blueberry Using Small RNA and Degradome Sequencing.
复制标题
使用小 RNA 和降解组测序比较分析蓝莓中与果实成熟相关的 miRNA 及其靶标
DOI：
10.3390/ijms18122767
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发表时间：
2017-12-19
影响因子：
5.6
通讯作者：
Bian S
中科院分区：
文献类型：
--
作者：
Hou Y;Zhai L;Li X;Xue Y;Wang J;Yang P;Cao C;Li H;Cui Y;Bian S
MicroRNAs(MiRNAs)在果实发育和成熟过程中起着重要的调节作用。蓝莓是一种重要的小型浆果类果树，具有经济价值和营养价值。然而，关于miRNAs及其参与蓝莓果实成熟的靶标的研究尚不清楚。本研究利用高通量测序技术，在代表果实成熟开始和成熟过程中的白果(WF)和蓝果(BF)文库中分别鉴定了28个家族84个已知miRNAs和16个新的miRNAs。其中41个miRNAs在果实成熟过程中差异表达，并进一步选取16个家族的16个miRNAs进行茎环qRT-PCR验证。同时，利用降解基因组测序技术对WF和BF文库中的41个已知miRNAs和7个新的miRNAs进行了178个靶点的鉴定，并用RLM-RACE(RNA Ligase-Mediated(RLM)-Rapid Amplify of cDNA Ends)方法验证了miR160的靶点。此外，还检测了6个miRNAs及其靶标在果实发育和成熟过程中的表达模式。最后，对miRNAs及其靶标的整合分析揭示了一个复杂的miRNA-mRNA调控网络，涉及广泛的生物过程。这些发现将有助于未来研究miRNA介导的调控蓝莓果实发育和成熟的机制。
MicroRNAs (miRNAs) play vital roles in the regulation of fruit development and ripening. Blueberry is an important small berry fruit crop with economical and nutritional value. However, nothing is known about the miRNAs and their targets involved in blueberry fruit ripening. In this study, using high-throughput sequencing of small RNAs, 84 known miRNAs belonging to 28 families and 16 novel miRNAs were identified in white fruit (WF) and blue fruit (BF) libraries, which represent fruit ripening onset and in progress, respectively. Among them, 41 miRNAs were shown to be differentially expressed during fruit maturation, and 16 miRNAs representing 16 families were further chosen to validate the sRNA sequencing data by stem-loop qRT-PCR. Meanwhile, 178 targets were identified for 41 known and 7 novel miRNAs in WF and BF libraries using degradome sequencing, and targets of miR160 were validated using RLM-RACE (RNA Ligase-Mediated (RLM)-Rapid Amplification of cDNA Ends) approach. Moreover, the expression patterns of 6 miRNAs and their targets were examined during fruit development and ripening. Finally, integrative analysis of miRNAs and their targets revealed a complex miRNA-mRNA regulatory network involving a wide variety of biological processes. The findings will facilitate future investigations of the miRNA-mediated mechanisms that regulate fruit development and ripening in blueberry.
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DOI：
10.3390/molecules20059326
发表时间：
2015-05-21
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Fortes AM;Teixeira RT;Agudelo-Romero P
通讯作者：
Agudelo-Romero P
影响因子：
14.9
作者：
Brousse C;Liu Q;Beauclair L;Deremetz A;Axtell MJ;Bouché N
通讯作者：
Bouché N
影响因子：
3.7
作者：
Harvey JJ;Lewsey MG;Patel K;Westwood J;Heimstädt S;Carr JP;Baulcombe DC
通讯作者：
Baulcombe DC
影响因子：
5.8
作者：
Addo-Quaye, Charles;Miller, Webb;Axtell, Michael J.
通讯作者：
Axtell, Michael J.
影响因子：
6.9
作者：
Boettcher, Christine;Keyzers, Robert A.;Davies, Christopher
通讯作者：
Davies, Christopher