Significantly elevated expression of PF4 (platelet factor 4) and eotaxin in the NOA mouse, a model for atopic dermatitis
Significantly elevated expression of PF4 (platelet factor 4) and eotaxin in the NOA mouse, a model for atopic dermatitis
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NOA 小鼠（特应性皮炎模型）中 PF4（血小板因子 4）和嗜酸细胞活化趋化因子的表达显着升高
DOI：
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发表时间：
1999
影响因子：
3.5
通讯作者：
Yusuke Nakamura
中科院分区：
文献类型：
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作者：
Otsu Watanabe;K. Natori;M. Tamari;Y. Shiomoto;S. Kubo;Yusuke Nakamura
NOA(Naruto Research Institute Otsuka Atrichia)小鼠是一种过敏性或特应性皮炎的动物模型，表现为皮肤溃烂病变，伴随着肥大细胞和嗜酸性粒细胞的聚集，血清IgE水平显著升高，以及抓挠行为。为了研究这种小鼠皮炎病理过程中的遗传因素，我们通过差异显示的方法，寻找在NOA小鼠的脾中与对照组相比表达不同的基因。我们克隆并鉴定了一个基因，该基因在NOA小鼠中的表达显著高于对照品系。该基因全长570个核苷酸，其中开放阅读框315个核苷酸，编码105个氨基酸。推导的氨基酸序列证实该基因为大鼠和人血小板因子(PF)4S的小鼠同源基因(在105个氨基酸中，同源性分别为89%和%)。Pf4是一种肝素结合蛋白，从活化的血小板的γ颗粒中释放出来，属于含有CxC基序的趋化因子分子家族。我们的结果提示，PF4的高表达可能在变态反应性皮炎的发病机制中起重要作用。
AbstractThe NOA (Naruto Research Institute Otsuka Atrichia) mouse, an animal model of allergic or atopic dermatitis, exhibits ulcerative skin lesions associated with accumulation of mast cells and eosinophils, a significantly increased level of serum IgE, and scratching behavior. To investigate genetic contributors to the pathological process of dermatitis in this murine model, we looked for genes that were expressed differently in spleens of NOA mice compared with controls, by means of a differential display method. We cloned and characterized one gene that revealed a significantly higher expression in the NOA mouse than in control strains. Its cDNA consisted of 570 nucleotides, including 315 nucleotides of open reading frame encoding 105 amino acids. The deduced amino acid sequence identified this gene as the murine homologue of rat and human platelet factor (PF) 4s (89% identity and 64% identity in 105 amino acids, respectively). PF4 is a heparinbinding protein that is released from γ-granules of activated platelets and belongs to the family of chemokine molecules that contain a CXC motif. Our results suggested that increased expression of PF4 may play an important role in the etiology of allergic dermatitis.
源自人红白血病细胞系的血小板因子 4 cDNA 的克隆和表征。
影响因子：
20.3
作者：
Poncz,M;Surrey,S;LaRocco,P;Weiss,MJ;Rappaport,EF;Conway,TM;Schwartz,E
通讯作者：
Schwartz,E
影响因子：
56.9
作者：
MARSH, DG;NEELY, JD;BEATY, TH
通讯作者：
BEATY, TH