Whole-genome DNA methylation profile of the jewel wasp (Nasonia vitripennis).
Whole-genome DNA methylation profile of the jewel wasp (Nasonia vitripennis).
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DOI：
10.1534/g3.113.008953
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发表时间：
2014-03-20
期刊：
影响因子：
--
通讯作者：
Drewell RA
中科院分区：
文献类型：
--
作者：
Beeler SM;Wong GT;Zheng JM;Bush EC;Remnant EJ;Oldroyd BP;Drewell RA
DNA甲基化的表观遗传标记，即在胞嘧啶残基上添加甲基（CH3），已在许多哺乳动物基因组中得到广泛研究，尽管它常见于基因的启动子区域，但它也涉及许多不同的生物学功能。在其他复杂的动物中，例如群居性昆虫，DNA甲基化已被确定与种姓分化有关，并且主要发生在基因体中。然而，甲基化在非社会性昆虫中的作用尚未得到彻底探索。在这里，我们展示了非社会性膜翅目宝石黄蜂 (Nasonia vitripennis) 的全基因组 DNA 甲基化谱。通过对从雄性 Nasonia thoraces 中提取的亚硫酸氢盐转化的 gDNA 进行高通量测序，我们能够确定整个基因组中哪些胞嘧啶残基被甲基化。我们发现绝大多数甲基化位点 (99.7%) 发生在胞嘧啶上，随后是 3' 方向的鸟嘌呤（CpG 位点）。此外，我们发现 Nasonia 的大部分甲基化发生在基因组的外显子区域内（超过 62%）。总体而言，Nasonia 中的甲基化很少，仅发生在所有位点的 0.18% 和 CpG 的 0.63% 处。我们对 Nasonia 甲基化组的分析表明，与哺乳动物中常见的甲基化谱相比，甲基化是稀疏的，并且主要局限于外显子。这种甲基化谱与社会膜翅目蜜蜂（Apis mellifera）的甲基化谱更相似。通过介绍 Nasonia 甲基化组，我们希望促进未来对社会性和非社会性膜翅目 DNA 甲基化调节功能的研究。
The epigenetic mark of DNA methylation, the addition of a methyl (CH3) group to a cytosine residue, has been extensively studied in many mammalian genomes and, although it is commonly found at the promoter regions of genes, it is also involved in a number of different biological functions. In other complex animals, such as social insects, DNA methylation has been determined to be involved in caste differentiation and to occur primarily in gene bodies. The role of methylation in nonsocial insects, however, has not yet been explored thoroughly. Here, we present the whole-genome DNA methylation profile of the nonsocial hymenopteran, the jewel wasp (Nasonia vitripennis). From high-throughput sequencing of bisulfite-converted gDNA extracted from male Nasonia thoraces, we were able to determine which cytosine residues are methylated in the entire genome. We found that an overwhelming majority of methylated sites (99.7%) occur at cytosines followed by a guanine in the 3′ direction (CpG sites). Additionally, we found that a majority of methylation in Nasonia occurs within exonic regions of the genome (more than 62%). Overall, methylation is sparse in Nasonia, occurring only at 0.18% of all sites and at 0.63% of CpGs. Our analysis of the Nasonia methylome revealed that in contrast to the methylation profile typically seen in mammals, methylation is sparse and is constrained primarily to exons. This methylation profile is more similar to that of the social hymenopteran species, the honey bee (Apis mellifera). In presenting the Nasonia methylome, we hope to promote future investigation of the regulatory function of DNA methylation in both social and nonsocial hymenoptera.
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影响因子：
16.8
作者：
Drewell, Robert A.;Lo, Nathan;Oldroyd, Benjamin P.
通讯作者：
Oldroyd, Benjamin P.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3
作者：
Xi Y;Chen YP;Cao M;Wang W;Wang F
通讯作者：
Wang F
影响因子：
64.8
作者：
Altshuler, D;Pollara, VJ;Lander, ES
通讯作者：
Lander, ES
在蜜蜂行为子播种中的表观遗传状态之间可逆切换。
影响因子：
25
作者：
Herb, Brian R.;Wolschin, Florian;Hansen, Kasper D.;Aryee, Martin J.;Langmead, Ben;Irizarry, Rafael;Amdam, Gro V.;Feinberg, Andrew P.
通讯作者：
Feinberg, Andrew P.