Dorsal root ganglion neurons innervating pelvic organs in the mouse express tyrosine hydroxylase.
Dorsal root ganglion neurons innervating pelvic organs in the mouse express tyrosine hydroxylase.
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DOI：
10.1016/j.neuroscience.2012.07.043
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发表时间：
2012-10-25
期刊：
影响因子：
3.3
通讯作者：
Gebhart, G. F.
中科院分区：
文献类型：
--
作者：
Brumovsky, P. R.;La, J-H.;McCarthy, C. J.;Hokfelt, T.;Gebhart, G. F.
以前在大鼠和小鼠的研究表明，支配非内脏组织的背根节(DRG)神经元亚群表达酪氨酸羟基酶(TH)。在这里，我们研究了用快速蓝(FB)逆行追踪的小鼠结肠或膀胱DRG神经元是否也表达免疫组织化学可检测到的TH。分析对象包括腰交感神经链(LSC)和盆腔大神经节(MPG)。使用先前鉴定的抗TH、去甲肾上腺素转运体1(Net-1)和降钙素基因相关肽(CGRP)的抗体。平均而言，14%的结直肠和17%的膀胱DRG神经元表达TH，几乎覆盖了所有大小的神经元，尽管更多的是在中等到小的范围内。此外，它们在腰椎的DRG中比在胸腰椎的DRG中更丰富，并且经常共表达CGRP。我们还在LSC和MPG中检测到几个TH免疫反应阳性(IR)的结直肠和膀胱神经元，前者更为常见。在背根节内未检测到Net-1-IR神经元，而在LSC和MPG中的大多数FB标记的TH-IR神经元共同表达该标记(与大多数其他未标记的靶器官THIR神经元一样)。TH-IR神经纤维分布于大肠和膀胱壁的各层，部分也可到达基底层粘膜细胞。这些器官中的大多数TH-IR纤维缺乏CGRP。综上所述，我们表明：1)之前未被描述的大肠和膀胱DRG神经元群表达TH，通常是CGRP，但不表达Net-1，这表明缺乏去甲肾上腺素能表型；2)结肠或膀胱中的TH-IR轴突/终末，自然地被认为来自自主神经来源，也可能来自感觉神经元。
Previous studies in rat and mouse documented that a subpopulation of dorsal root ganglion (DRG) neurons innervating non-visceral tissues express tyrosine hydroxylase (TH). Here we studied whether or not mouse DRG neurons retrogradely traced with Fast Blue (FB) from colorectal or urinary bladder also express immunohistochemically detectable TH. The lumbar sympathetic chain (LSC) and major pelvic ganglion (MPG) were included in the analysis. Previously characterized antibodies against TH, norepinephrine transporter type 1 (NET-1) and calcitonin gene-related peptide (CGRP) were used. On average, 14% of colorectal and 17 % of urinary bladder DRG neurons expressed TH and spanned virtually all neuronal sizes, although more often in the medium-sized to small ranges. Also, they were more abundant in lumbosacral than thoracolumbar DRGs, and often coexpressed CGRP. We also detected several TH-immunoreactive (IR) colorectal and urinary bladder neurons in the LSC and the MPG, more frequently in the former. No NET-1-IR neurons were detected in DRGs, whereas the majority of FB-labeled, TH-IR neurons in the LSC and MPG coexpressed this marker (as did most other THIR neurons not labeled from the target organs). TH-IR nerve fibers were detected in all layers of the colorectum and the urinary bladder, with some also reaching the basal mucosal cells. Most TH-IR fibers in these organs lacked CGRP. Taken together, we show: 1) that a previously undescribed population of colorectal and urinary bladder DRG neurons expresses TH, often CGRP but not NET-1, suggesting absence of a noradrenergic phenotype; and 2) that TH-IR axons/terminals in colon or urinary bladder, naturally expected to derive from autonomic sources, could also originate from sensory neurons.
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DOI：
10.1016/s0165-1838(97)00032-5
发表时间：
1997-07-14
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
CallsenCencic, P;Mense, S
通讯作者：
Mense, S
影响因子：
29.4
作者：
Brierley, SM;Jones, RCW;Blackshaw, LA
通讯作者：
Blackshaw, LA
影响因子：
5.5
作者：
Brierley, SM;Carter, R;Blackshaw, LA
通讯作者：
Blackshaw, LA
影响因子：
3.4
作者：
Dina, Olayinka A.;Khasar, Sachia G.;Levine, Jon D.
通讯作者：
Levine, Jon D.
影响因子：
2.5
作者：
Herradon, Gonzalo;Ezquerra, Laura;Alguacil, Luis F.
通讯作者：
Alguacil, Luis F.