Manipulation of the rice L-galactose pathway: evaluation of the effects of transgene overexpression on ascorbate accumulation and abiotic stress tolerance.
Manipulation of the rice L-galactose pathway: evaluation of the effects of transgene overexpression on ascorbate accumulation and abiotic stress tolerance.
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水稻 L-半乳糖途径的操纵：评估转基因过表达对抗坏血酸积累和非生物胁迫耐受性的影响
DOI：
10.1371/journal.pone.0125870
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liu QQ
中科院分区：
文献类型：
--
作者：
Zhang GY;Liu RR;Zhang CQ;Tang KX;Sun MF;Yan GH;Liu QQ
抗坏血酸（ASA）是植物中最丰富的水溶性抗氧化剂，它在植物生长，发育和非生物胁迫耐受性中起着至关重要的作用。在本研究中，六个关键的拟南芥或菜籽基因在ASA生物合成中涉及的六个主要的拟南芥在一个精英Japonica水稻品种中构成过度表达。 These genes encoded the GDP-mannose pyrophosphorylase (GMP), GDP-mannose-3',5'-epimerase (GME), GDP-L-galactose phosphorylase (GGP), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH), and L-galactono-1,4-lactone脱氢酶（GALDH）。仔细评估了转基因表达对稻叶ASA积累的影响。在纯合转基因幼苗中，ATGGP转基因线具有最高的ASA含量（比空载体转基因控制大2.55倍），其次是ATGME和ATGDH转基因线。此外，除了ATGPP线外，ASA含量的增加也引起了氧化还原状态的增加（ASA/DHA比）。为了评估盐耐受性，将ATGGP和ATGME转基因幼苗暴露于盐胁迫中一周。与对照工厂相比，转基因线的相对植物高度，根长和新鲜重量生长速率明显更高。总而言之，我们的结果表明，GGP可能是稻米ASA生物合成的关键限制步骤，而ASA含量升高的植物表现出对盐胁迫的耐受性的增强。
Ascorbic acid (AsA) is the most abundant water-soluble antioxidant in plants, and it plays a crucial role in plant growth, development and abiotic stress tolerance. In the present study, six key Arabidopsis or rapeseed genes involved in AsA biosynthesis were constitutively overexpressed in an elite Japonica rice cultivar. These genes encoded the GDP-mannose pyrophosphorylase (GMP), GDP-mannose-3',5'-epimerase (GME), GDP-L-galactose phosphorylase (GGP), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH), and L-galactono-1,4-lactone dehydrogenase (GalLDH). The effects of transgene expression on rice leaf AsA accumulation were carefully evaluated. In homozygous transgenic seedlings, AtGGP transgenic lines had the highest AsA contents (2.55-fold greater than the empty vector transgenic control), followed by the AtGME and AtGDH transgenic lines. Moreover, with the exception of the AtGPP lines, the increased AsA content also provoked an increase in the redox state (AsA/DHA ratio). To evaluate salt tolerance, AtGGP and AtGME transgenic seedlings were exposed to salt stress for one week. The relative plant height, root length and fresh weight growth rates were significantly higher for the transgenic lines compared with the control plants. Altogether, our results suggest that GGP may be a key rate-limiting step in rice AsA biosynthesis, and the plants with elevated AsA contents demonstrated enhanced tolerance for salt stress.
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影响因子：
4.3
作者：
Hoeller, Stefanie;Hajirezaei, Mohammad-Reza;Frei, Michael
通讯作者：
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影响因子：
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作者：
Fukunaga, Kazunari;Fujikawa, Yukichi;Esaka, Muneharu
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Hemavathi;Upadhyaya, Chandrama Prakash;Park, Se Won
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影响因子：
3.1
作者：
Hemavathi;Upadhyaya, Chandrama Prakash;Park, Se Won
通讯作者：
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影响因子：
7.2
作者：
Gatzek, S;Wheeler, GL;Smirnoff, N
通讯作者：
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