Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production
Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production
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罗氏链霉菌 SCSIO 11720 嗜热血红蛋白降解蛋白酶的表征及其在抗菌肽生产中的应用
DOI：
10.1007/s12257-013-0771-9
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发表时间：
2015-03
影响因子：
3.2
通讯作者：
Si Zhang
中科院分区：
文献类型：
--
作者：
Jian Yang;Jie Li;Yunfeng Hu;Lizhen Li;Lijuan Long;Fazuo Wang;Si Zhang
对屠宰场副产品进行高值化利用，是一项经济效益和环境效益并重的工程。血红蛋白降解蛋白酶PA 720是从红滨藻相关菌株鲁格链霉菌（Streptomyces rutgersensis）SCSIO 11720（SCSIO 11720）的发酵液中纯化的，并且在70°C、pH 10.5下表现出最大活性。对分离菌株进行基因型鉴定，并将16 s RNA基因序列登录于GenBank，登录号为KC 414842。动力学和热力学研究表明，该酶的失活活化能（Ea）、ΔG*（自由能）、ΔH*（焓）和ΔS*（熵）分别为62.71 kJ/mol、74.26 kJ/mol、59.86 kJ/mol和−41.97 J/mol/K。当使用酪蛋白（9，953 U/mg）和血红蛋白（9，854 U/mg）作为底物时，观察到蛋白酶PA 720的最高比活性。 根据PA 720的部分氨基酸序列，通过简并引物PCR获得了编码该蛋白酶的全基因，并已在GenBank中保藏，保藏号为KC 414842。推导的氨基酸序列与枯草杆菌蛋白酶家族中的其它嗜热蛋白酶具有同源性。
High value utilization of byproduct of slaughterhouses is regarded as beneficial to both economy and environment. A hemoglobin degrading protease PA720 was purified from fermentation broth of a Porites lutea associated strain Streptomyces rutgersensis SCSIO 11720 (SCSIO 11720) and exhibited maximal activity at 70°C, pH 10.5. The isolated strain was identified by genotypic method and 16 s RNA gene sequence was deposited in GenBank under accession No. KC414842. The enzyme exhibited excellent thermostability since it was stable at 70°C with t1/2 value of 1,386.3 min. Kinetic and thermodynamic study showed the activation energy (Ea), ΔG* (free energy), ΔH* (enthalpy) and ΔS* (entropy) for protease deactivation were 62.71 kJ/mol, 74.26 kJ/mol, 59.86 kJ/mol, and −41.97 J/mol/K, respectively. Highest specific activity of protease PA720 was observed when using casein (9,953 U/mg) and hemoglobin (9,854 U/mg) as substrates. Based on partial amino acid sequences of the enzyme PA720, the full gene encoding this protease was obtained by degenerate primer PCR and has been deposited in GenBank under accession No. KC414842. The deduced amino acid sequence exhibits homology with other microbial thermophilic proteases in subtilisin family.
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影响因子：
4.1
作者：
James Ze Wang;M. Zhang;F. Ren;B. Han;L. Wang;S. W. Chen;A. Humera
通讯作者：
James Ze Wang;M. Zhang;F. Ren;B. Han;L. Wang;S. W. Chen;A. Humera
DOI：
10.1099/ijs.0.035402-0
发表时间：
2012-07
影响因子：
2.8
作者：
Jie Li;Jian Yang;Wen-Yong Zhu;Jie He;Xin-Peng Tian;Qiong Xie;Si Zhang;Wenjun Li
通讯作者：
Jie Li;Jian Yang;Wen-Yong Zhu;Jie He;Xin-Peng Tian;Qiong Xie;Si Zhang;Wenjun Li
DOI：
10.1099/ijs.0.035386-0
发表时间：
2012-10
影响因子：
2.8
作者：
Ying Xu;Jie He;Xin-Peng Tian;Jie Li;Ling-Ling Yang-Ling;Qiong Xie;Shukun Tang;Yi-Guang Chen;Si Zhang;Wenjun Li
通讯作者：
Ying Xu;Jie He;Xin-Peng Tian;Jie Li;Ling-Ling Yang-Ling;Qiong Xie;Shukun Tang;Yi-Guang Chen;Si Zhang;Wenjun Li
影响因子：
0.6
作者：
P. Mitra;P. K. Chakrabartty
通讯作者：
P. Mitra;P. K. Chakrabartty
影响因子：
6.4
作者：
Turner P;Mamo G;Karlsson EN
通讯作者：
Karlsson EN