Potential and utilization of thermophiles and thermostable enzymes in biorefining.
Potential and utilization of thermophiles and thermostable enzymes in biorefining.
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DOI：
10.1186/1475-2859-6-9
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发表时间：
2007-03-15
影响因子：
6.4
通讯作者：
Karlsson EN
中科院分区：
文献类型：
--
作者：
Turner P;Mamo G;Karlsson EN
在当今世界，在不同的生物精炼厂中使用可再生的、廉价的和容易获得的生物质来生产各种精细和散装化学品的趋势越来越大。生物精炼厂利用微生物细胞及其酶的活性将生物质转化为目标产品。这些方法中的许多需要在高温下操作稳定的酶，从而允许例如容易混合、更好的底物溶解度、高传质速率和降低的污染风险。嗜热菌通常被认为是工业上相关的热稳定酶的来源。在这里，我们讨论了现有的和潜在的应用嗜热菌和热稳定的酶，重点是转化含碳水化合物的原料。它们在生物精炼中的重要性是用木质纤维素和淀粉转化为所需产品的例子来解释的。还评估了增强酶在体内和体外的热稳定性的策略。此外，本文还综述了在嗜热宿主中表达重组酶的载体的研究进展。
In today's world, there is an increasing trend towards the use of renewable, cheap and readily available biomass in the production of a wide variety of fine and bulk chemicals in different biorefineries. Biorefineries utilize the activities of microbial cells and their enzymes to convert biomass into target products. Many of these processes require enzymes which are operationally stable at high temperature thus allowing e.g. easy mixing, better substrate solubility, high mass transfer rate, and lowered risk of contamination. Thermophiles have often been proposed as sources of industrially relevant thermostable enzymes. Here we discuss existing and potential applications of thermophiles and thermostable enzymes with focus on conversion of carbohydrate containing raw materials. Their importance in biorefineries is explained using examples of lignocellulose and starch conversions to desired products. Strategies that enhance thermostablity of enzymes both in vivo and in vitro are also assessed. Moreover, this review deals with efforts made on developing vectors for expressing recombinant enzymes in thermophilic hosts.
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影响因子：
4.4
作者：
Burhan, A;Nisa, U;Osman, G
通讯作者：
Osman, G
影响因子：
4.1
作者：
Bolam, DN;Ciruela, A;Gilbert, HJ
通讯作者：
Gilbert, HJ
影响因子：
5
作者：
Bjornsdottir, SH;Thorbjarnardottir, SH;Eggertsson, G
通讯作者：
Eggertsson, G
影响因子：
3.4
作者：
Birgisson, H;Hreggvidsson, GO;Mattiasson, B
通讯作者：
Mattiasson, B
影响因子：
3.2
作者：
BROCK, TD;FREEZE, H
通讯作者：
FREEZE, H