Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici).
Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici).
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DOI：
10.1186/1471-2164-8-145
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发表时间：
2007-06-04
期刊：
影响因子：
4.4
通讯作者：
Campbell KG
中科院分区：
文献类型：
--
作者：
Ling P;Wang M;Chen X;Campbell KG
条锈菌(Puccinia Striiformis)是一种引起条锈病的植物病原真菌，是世界范围内危害农作物和牧草的重要病害之一。然而，对其基因组和参与病原菌生物学和致病性的基因知之甚少。我们构建了该真菌的全长cDNA，并通过与其他真菌中已报道的基因进行序列比较，确定了第一组基因的功能。以条斑病菌PST-78小种的夏孢子为材料，构建了由42,240个克隆组成的全长cDNA文库，平均插入片段为1.9 kb。小黑麦。从测序的196个克隆中，我们确定了73个克隆的功能(37.2%)。此外，36个克隆(18.4%)与假设的蛋白质有显著同源性，37个克隆(18.9%)与其他真菌的基因有一定的同源性，其余50个克隆(25.5%)不产生任何HIT。从73个具有功能的克隆中，我们鉴定了51个不同的编码蛋白质产物的基因，涉及氨基酸代谢、细胞防御、细胞周期、细胞信号、细胞结构和生长、能量周期、脂和核苷酸代谢、蛋白质修饰、核糖体蛋白复合体、糖代谢、转录因子、运输代谢和毒力/感染。该全长cDNA文库可用于鉴定条斑病菌的功能基因。
Puccinia striiformis is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little is know about its genome and genes involved in the biology and pathogenicity of the pathogen. We initiated the functional genomic research of the fungus by constructing a full-length cDNA and determined functions of the first group of genes by sequence comparison of cDNA clones to genes reported in other fungi. A full-length cDNA library, consisting of 42,240 clones with an average cDNA insert of 1.9 kb, was constructed using urediniospores of race PST-78 of P. striiformis f. sp. tritici. From 196 sequenced cDNA clones, we determined functions of 73 clones (37.2%). In addition, 36 clones (18.4%) had significant homology to hypothetical proteins, 37 clones (18.9%) had some homology to genes in other fungi, and the remaining 50 clones (25.5%) did not produce any hits. From the 73 clones with functions, we identified 51 different genes encoding protein products that are involved in amino acid metabolism, cell defense, cell cycle, cell signaling, cell structure and growth, energy cycle, lipid and nucleotide metabolism, protein modification, ribosomal protein complex, sugar metabolism, transcription factor, transport metabolism, and virulence/infection. The full-length cDNA library is useful in identifying functional genes of P. striiformis.
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