Short-term effects of mechanical loading on the transdifferentiation of condylar chondrocytes.
Short-term effects of mechanical loading on the transdifferentiation of condylar chondrocytes.
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DOI：
10.1016/j.ajodo.2022.12.011
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发表时间：
2023-08
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影响因子：
--
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中科院分区：
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--
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软骨细胞向骨细胞的转分化解释了大多数产前和产后早期髁突生长，但其在产后后期生长中的作用和调节转分化的机制尚不清楚。本研究旨在量化机械载荷对出生后晚期髁突生长过程中软骨细胞衍生成骨的影响，采用短期下颌侧突模型。将34只4周龄agrecan - creert2、R26RtdTomato、2.3Col1a1-GFP复合小鼠注射他莫昔芬，分为对照组和实验组。粘接矫治器移位实验小鼠下颌骨5天，造成右髁和左髁分别前突和后缩。x线摄影，显微计算机断层摄影和组织形态学分析进行。实验组和对照组均出现软骨细胞向骨细胞的大量转分化。实验小鼠下颌骨发育不对称，下颌骨前突侧明显长于后突侧。突出髁的软骨形成明显增加，软骨细胞来源的成骨细胞数量增加，尤其是在后三分之一。在后退侧则出现相反的效果。软骨细胞向骨细胞的转分化发生在出生后晚期的髁突生长期间。侧突调节髁突软骨形成和软骨细胞转分化，改变髁突生长的数量和方向。我们的研究表明，软骨细胞是髁突骨形成的关键角色，应该成为控制和进一步了解髁突生长的研究重点。
Transdifferentiation of chondrocytes into bone cells explains most of the prenatal and early postnatal condylar growth, but its role during later postnatal growth and the mechanisms regulating transdifferentiation remain unknown. This study aimed to quantify the effects of mechanical loading on chondrocyte-derived osteogenesis during late postnatal condylar growth using a short-term mandibular laterotrusion model. Thirty 4-week-old Aggrecan-CreERT2, R26RtdTomato, and 2.3Col1a1-GFP compound mice received tamoxifen injections and were divided into control and experimental groups. Appliances were bonded to shift the mandibles of the experimental mice for 5 days, causing protrusion and retrusion of the right and left condyles, respectively. Radiographic, microcomputed tomographic, and histomorphometric analyses were performed. The experimental and control groups showed substantial transdifferentiation of chondrocytes into bone cells. The experimental mice developed asymmetric mandibles, with the protrusive side significantly longer than the retrusive side. The protrusive condyles showed significantly increased chondrogenesis and greater numbers of chondrocyte-derived osteogenic cells, especially in the posterior third. The opposite effects were seen on the retrusive side. Transdifferentiation of chondrocytes into bone cells occurs during late postnatal condylar growth. Laterotrusion regulates condylar chondrogenesis and chondrocyte transdifferentiation, which alters the amount and direction of condylar growth. Our study demonstrated that chondrocytes are key players in condylar bone formation and should be the focus of studies to control and further understand condylar growth.
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影响因子：
64.5
作者：
Kretzschmar, Kai;Watt, Fiona M.
通讯作者：
Watt, Fiona M.
DOI：
10.1016/j.ajodo.2006.10.032
发表时间：
2008-12-01
影响因子：
3
作者：
Ishii, Takenobu;Yamaguchi, Hideharu
通讯作者：
Yamaguchi, Hideharu
影响因子：
1.2
作者：
Jing, Yan;Hinton, Robert J.;Feng, Jian Q.
通讯作者：
Feng, Jian Q.
软骨发生和成骨是一种连续的发育和谱系定义的生物学过程。
影响因子：
4.6
作者：
Jing Y;Jing J;Ye L;Liu X;Harris SE;Hinton RJ;Feng JQ
通讯作者：
Feng JQ
影响因子：
3
作者：
Lin H;Zhu P;Lin Y;Wan S;Shu X;Xu Y;Zheng Y
通讯作者：
Zheng Y