The biometric measurement of alcohol consumption.
The biometric measurement of alcohol consumption.
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酒精消耗的生物识别测量。
DOI：
10.1111/j.1530-0277.2011.01605.x
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
WHO/ISBRAStudyonStateandTraitMarkersof
中科院分区：
文献类型：
--
作者：
Snell,LawrenceD;Ramchandani,VijayA;Saba,Laura;Herion,David;Heilig,Markus;George,DavidT;Pridzun,Lutz;Helander,Anders;Schwandt,MelanieL;Phillips,MonteJ;Hoffman,PaulaL;Tabakoff,Boris;WHO/ISBRAStudyonStateandTraitMarkersof
背景：适当确定个人的饮酒史是疾病医学诊断的重要组成部分，并影响适当治疗策略的实施，包括药物处方，以及对危险/有害饮酒水平的负面身体和社会后果的干预。继续寻求生物（生物计量）诊断测试，以提供在器官损害发生之前个人当前和过去饮酒数量和频率的信息。方法：对2组有不同水平饮酒史的受试者进行血小板单胺氧化酶B （MAO‐B）蛋白测定。用免疫印迹法或ELISA法检测。开发和评估新的基于ELISA的血小板MAO - B蛋白水平的测量描述。结果：一组受试者为非寻求治疗的横断面受试者样本，另一组受试者为纵向随访的住院组。一种将血小板MAO - B蛋白与血浆碳水化合物缺乏转铁蛋白（CDT）水平和肝酶（天冬氨酸转氨酶或γ -谷氨酰转移酶[GGT]）相结合的算法可以在横切性不寻求治疗的人群中以最高的灵敏度和特异性检测危险/有害酒精使用（HHAU）。在寻求治疗的人群中，入院时低MAO - B蛋白水平与入院前大量饮酒有关，并且这些蛋白水平在戒酒一段时间后增加。结论：血小板MAO - B蛋白测定对男性饮酒者特别有效。然而，MAO - B蛋白测量与CDT和GGT测量联合使用确实提高了这两种标记物在确定女性HHAU中的诊断效用。此外，在酒精依赖治疗期间测量血小板MAO - B蛋白水平的变化可能有助于监测治疗方案的成功。
Background:Proper ascertainment of the history of alcohol consumption by an individual is an important component of medical diagnosis of disease and influences the implementation of appropriate treatment strategies that include prescription of medication, as well as intervention for the negative physical and social consequences of hazardous/harmful levels of alcohol consumption. Biological (biometric) diagnostic tests that provide information on current and past quantity and frequency of alcohol consumption by an individual, prior to onset of organ damage, continue to be sought.Methods:Platelet monoamine oxidase B (MAO‐B) protein was quantitated in 2 populations of subjects who had histories of different levels of alcohol consumption. Levels were assayed by immunoblotting or by ELISA. The development and evaluation of the new ELISA‐based measure of platelet MAO‐B protein levels is described.Results:One subject population constituted a nontreatment‐seeking, cross‐sectional subject sample, and the other population was a longitudinally followed, hospitalized group of subjects. An algorithm combining measures of platelet MAO‐B protein with the plasma levels of carbohydrate‐deficient transferrin (CDT) and with liver enzymes (aspartate aminotransferase or γ‐glutamyltransferase [GGT]) can detect hazardous/harmful alcohol use (HHAU) with the highest sensitivity and specificity in the cross‐sectional nontreatment‐seeking population. In the treatment‐seeking population, low MAO‐B protein levels at admission are associated with heavy drinking prior to admission, and these protein levels increase over a period of abstinence from alcohol.Conclusions:The platelet MAO‐B protein measurement is particularly effective for male alcohol consumers. The combined use of MAO‐B protein measures together with measures of CDT and GGT does, however, improve the diagnostic utility of both markers for ascertaining HHAU in women. Furthermore, measurement of changes in platelet MAO‐B protein levels during treatment for alcohol dependence may help monitor the success of the treatment program.
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DOI：
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发表时间：
1983
期刊：
影响因子：
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作者：
C. Walker;G. Streisinger
通讯作者：
G. Streisinger
与细菌氯霉素乙酰转移酶基因相关的鼠αA晶状体蛋白、鼠α2(I)胶原蛋白或禽肉瘤病毒基因的启动子序列直接指导转基因小鼠中氯霉素乙酰转移酶表达的组织特异性模式。
DOI：
--
发表时间：
1985
期刊：
Cold Spring Harbor Symposia on Quantitative Biology
影响因子：
--
作者：
Heiner Westphal;Paul A. Overbeek;J. Khillan;A. B. Chepelinsky;A. Schmidt;Kathleen A. Mahon;Kenneth E. Bernstein;J. Piatigorsky;B. Crombrugghe
通讯作者：
B. Crombrugghe
用兔 fB 珠蛋白基因转化青蛙胚胎
DOI：
--
发表时间：
--
期刊：
影响因子：
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作者：
S. Rusconi;W. Schaffner
通讯作者：
W. Schaffner
斑马鱼、斑马鱼的生物学及其在渔业研究中的应用。
DOI：
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发表时间：
1977
期刊：
影响因子：
--
作者：
H. Laale
通讯作者：
H. Laale
DOI：
10.1073/pnas.78.8.5051
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
RUSCONI, S;SCHAFFNER, W
通讯作者：
SCHAFFNER, W