Enhanced therapeutic efficacy of LHRHa-targeted brucea javanica oil liposomes for ovarian cancer.
Enhanced therapeutic efficacy of LHRHa-targeted brucea javanica oil liposomes for ovarian cancer.
复制标题
靶向 LHRHa 的鸦胆子油脂质体增强卵巢癌的治疗效果
DOI：
10.1186/s12885-016-2870-4
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发表时间：
2016-10-29
期刊：
影响因子：
3.8
通讯作者：
Chang S
中科院分区：
文献类型：
--
作者：
Ye H;Liu X;Sun J;Zhu S;Zhu Y;Chang S
BackgroundAlthough brucea javanica oil liposomes (BJOLs) have been used clinically to treat ovarian cancer, its clinical efficacy is often limited by systemic side effects due to non-specific distribution. Luteinizing hormone releasing hormone receptor (LHRHR) is overexpressed in most ovarian cancers but negligibly expressed in most of the other visceral organs. In this study, we aimed to develop a novel LHRHa targeted and BJO-loaded liposomes (LHRHa-BJOLs), and investigate its characteristics, targeting ability and anti-ovarian cancer efficiency both in vitro and in vivo.MethodsThe LHRHa-BJOLs were prepared by film-dispersion and biotin-streptavidin linkage methods, and characterized in terms of its morphology, particle size, zeta potential, ligand conjugation, encapsulation efficiency and stability. The targeting nature and antitumor effects of the liposomes were evaluated in vitro using cultured human ovarian cancer A2780/DDP cells, and in vivo using ovarian cancer-bearing nude mice.ResultsThe LHRHa-BJOLs were successfully synthesized, with a uniformly spherical shape, appropriate particle size and zeta potential, as well as a high encapsulation efficiency. Compared to non-targeted liposomes and BJO emulsion, the LHRHa-BJOLs could significantly increase specific intracellular uptaking rate, enhance cell inhibitory effect and induce cell apoptosis in A2780/DDP cells in vitro. Meanwhile, LHRHa-BJOLs also had a significantly stronger activity of targeting tumor tissue, inhibiting tumor growth, inducing tumor apoptosis and prolonging survival time in ovarian cancer-bearing mice in vivo.ConclusionsOur experiment suggests that LHRHa-BJOLs may be a useful targeted drug for the treatment of ovarian cancer.
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超声介导的 LHRHa 靶向和负载紫杉醇脂质微泡的破坏诱导卵巢癌细胞的增殖抑制和凋亡
影响因子：
4.9
作者：
Liu H;Chang S;Sun J;Zhu S;Pu C;Zhu Y;Wang Z;Xu RX
通讯作者：
Xu RX
影响因子：
2.6
作者：
Engel, J. B.;Schally, A. V.;Ortmann, O.
通讯作者：
Ortmann, O.
影响因子：
3.3
作者：
Cui, Yanxia;Wu, Zimei;Liu, Jianping
通讯作者：
Liu, Jianping
DOI：
10.1073/pnas.0504274102
发表时间：
2005-09-06
影响因子：
11.1
作者：
Dharap, SS;Wang, Y;Minko, T
通讯作者：
Minko, T
影响因子：
8.4
作者：
Chang, Shufang;Guo, Juan;Sun, Jiangchuan;Zhu, Shenyin;Yan, Yu;Zhu, Yi;Li, Min;Wang, Zhigang;Xu, Ronald X.
通讯作者：
Xu, Ronald X.