Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells.
Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells.
复制标题
DOI：
10.1016/j.ultsonch.2012.06.015
复制
发表时间：
2013-01
影响因子：
8.4
通讯作者：
Xu, Ronald X.
中科院分区：
文献类型：
--
作者：
Chang, Shufang;Guo, Juan;Sun, Jiangchuan;Zhu, Shenyin;Yan, Yu;Zhu, Yi;Li, Min;Wang, Zhigang;Xu, Ronald X.
超声靶向微泡破坏（UTMD）技术可用于非病毒基因治疗。以野生型p53（wtp 53）为靶点的肿瘤抑制基因可能为卵巢癌患者提供一种具有临床前景的治疗方法。然而，UTMD介导的基因治疗通常使用具有次优基因转染效率的非靶向微泡。我们合成了一种用于UTMD介导的wtp 53基因治疗卵巢癌细胞的靶向微泡剂。脂质微泡通过抗生物素蛋白-生物素连接与促黄体生成激素释放激素类似物（LHRHa）缀合以靶向表达LHRH受体的卵巢癌A2780/DDP细胞。将微泡与pEGFP-N1-wtp 53质粒混合。经1 MHz脉冲超声（0.5W/cm ~ 2）作用30 s后，wtp 53基因转染卵巢癌细胞。转染效率为（43.90 ± 6.19）%。转染后wtp 53 mRNA表达率为（97.08 ± 12.18）%。基因治疗后细胞凋亡率为（39.67 ± 5.95）%。与其他处理组相比，靶向微泡超声介导的转染效率和细胞凋亡率更高（p < 0.05）。我们的实验验证了超声介导靶向微泡可以提高卵巢癌细胞基因转染效率的假设。
Ultrasound-targeted microbubble destruction (UTMD) technique can be potentially used for non-viral delivery of gene therapy. Targeting wild-type p53 (wtp53) tumor suppressor gene may provide a clinically promising treatment for patients with ovarian cancer. However, UTMD mediated gene therapy typically uses non-targeted microbubbles with suboptimal gene transfection efficiency. We synthesized a targeted microbubble agent for UTMD mediated wtp53 gene therapy in ovarian cancer cells. Lipid micro-bubbles were conjugated with a Luteinizing Hormone–Releasing Hormone analog (LHRHa) via an avidin– biotin linkage to target the ovarian cancer A2780/DDP cells that express LHRH receptors. The microbubbles were mixed with the pEGFP-N1-wtp53 plasmid. Upon exposure to 1 MHz pulsed ultrasound beam (0.5 W/cm2) for 30 s, the wtp53 gene was transfected to the ovarian cancer cells. The transfection efficiency was (43.90 ± 6.19)%. The expression of wtp53 mRNA after transfection was (97.08 ± 12.18)%. The cell apoptosis rate after gene therapy was (39.67 ± 5.95)%. In comparison with the other treatment groups, ultrasound mediation of targeted microbubbles yielded higher transfection efficiency and higher cell apoptosis rate (p < 0.05). Our experiment verifies the hypothesis that ultrasound mediation of targeted microbubbles will enhance the gene transfection efficiency in ovarian cancer cells.
登录
查看更多内容
DOI：
10.1016/j.bbaexp.2003.12.003
发表时间：
2004-02-20
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Oberle, V;de Jong, G;Hoekstra, D
通讯作者：
Hoekstra, D
DOI：
10.1186/1756-9966-29-152
发表时间：
2010-11-23
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Chen ZY;Liang K;Qiu RX
通讯作者：
Qiu RX
影响因子：
4.4
作者：
Sonoda, S;Tachibana, K;Sakamoto, T
通讯作者：
Sakamoto, T
影响因子：
4.2
作者：
Gao, Zhonggao;Kennedy, Anne M.;Rapoport, Natalya Y.
通讯作者：
Rapoport, Natalya Y.
影响因子：
3
作者：
Felgner, PL
通讯作者：
Felgner, PL