Characterization of cytoplasmic factors which complement Ca2+ channel mutations in Paramecium tetraurelia.
Characterization of cytoplasmic factors which complement Ca2+ channel mutations in Paramecium tetraurelia.
复制标题
四尿草履虫中补充 Ca2 通道突变的细胞质因子的表征。
作者：
N. Haga;M. Forte;Y. Saimi;C. Kung
The analysis of Ca2+-channel function in the single-celled eukaryote Paramecium can be extended to a biochemical based on recent observations that transfer of cytoplasm from wild-type cells into mutants lacking Ca2+-channel function ("pawn" mutants) causes the mutant cells to regain Ca2-channel activity. Using a convenient behavioral assay for Ca2+-channel function, we have used microinjection of cytoplasmic fractions into mutant cells to enrich for and characterize those components from wild-type cytoplasm which can "cure" cells carrying mutations in the 3 different pawn genes affecting Ca2+-channel activity (pwA,pwB, and pwC). In each case, the curing factor appears to be a protein component of an intracellular membrane. They are distinguishable on the basis of thermal, pH and divalent ion sensitivities. In addition, the factor curing the pwC mutational defect has been purified more than 180-fold. Furthermore, the pwB curing activity appears to be amplified during sequential transfer between pwB cells.
DOI：
10.1073/pnas.77.12.7492
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CASTELLUCCI, VF;KANDEL, ER;GREENGARD, P
通讯作者：
GREENGARD, P
离子是否参与钙通道的门控？
DOI：
10.1126/science.6289432
发表时间：
1982
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Saimi,Y;Kung,C
通讯作者：
Kung,C