Effect of Clostridium butyricum and Butyrate on Intestinal Barrier Functions: Study of a Rat Model of Severe Acute Pancreatitis With Intra-Abdominal Hypertension.
Effect of Clostridium butyricum and Butyrate on Intestinal Barrier Functions: Study of a Rat Model of Severe Acute Pancreatitis With Intra-Abdominal Hypertension.
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丁酸梭菌和丁酸盐对肠屏障功能的影响：重症急性胰腺炎伴腹内高压大鼠模型的研究
DOI：
10.3389/fphys.2020.561061
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发表时间：
2020
影响因子：
4
通讯作者：
Wei B
中科院分区：
文献类型：
--
作者：
Zhao HB;Jia L;Yan QQ;Deng Q;Wei B
重症急性胰腺炎（SAP）与腹腔内高压（IAH）和腹腔间隔室综合征（ACS）有关，但这些疾病的治疗是困难的。我们研究了SAP + IAH大鼠模型，以确定口服丁酸梭菌及其主要代谢产物丁酸盐对肠屏障功能的影响。将48只大鼠分为4组，每组12只：假手术组（Sham）、SAP+IAH组、SAP+IAH+C组。butyricum和SAP + IAH + butyrate。采用胰胆管内注入牛磺胆酸钠诱导SAP模型，测定腹腔内压（IAP），24 h后处死动物。测定血浆中淀粉酶、二胺氧化酶（DAO）、异硫氰酸荧光素（FITC）-葡聚糖、肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-6、IL-1β、IL-12、脂多糖（LPS）等指标和粪便丁酸水平。采用组织学检查胰腺和小肠，RT-PCR和Western blotting检测小肠组织紧密连接蛋白（紧密连接蛋白ZO-1、claudin-1、claudin-2、occluding）、基质金属蛋白酶9（MMP 9）和TNF-α的表达。用16 S rRNA序列测定法检测大鼠肠道植物群。SAP + IAH的诱导改变了肠道屏障的几种功能，并增强了肠道通透性，降低了ZO-1，claudin-1，occludin的水平，微生物群落的丰富性和多样性，厚壁菌门的相对丰度（RA）和益生菌的数量。然而，SAP+IAH诱导增加了粪便中Claudin-2、MMP 9和TNF-α的表达，以及变形菌和病原体的RA。大鼠口服C。丁酸盐或丁酸盐降低了肠损伤和血浆DAO、LPS和炎性细胞因子水平。对SAP+IAH大鼠的这项研究表明，口服C。丁酸盐或丁酸盐可能通过改变肠粘膜屏障的功能而减少肠损伤。
Severe acute pancreatitis (SAP) is associated with intra-abdominal hypertension (IAH) and abdominal compartment syndrome (ACS), but treatment of these conditions is difficult. We studied a rat model of SAP + IAH to determine the effect of oral administration of Clostridium butyricum and butyrate (its major metabolite) on intestinal barrier functions. A total of 48 rats were assigned to four groups, with 12 rats per group: Sham, SAP+IAH, SAP+IAH+C. butyricum, and SAP + IAH + butyrate. SAP was induced by sodium taurocholate infusion into the biliopancreatic duct, intra-abdominal pressure (IAP), mortality was measured 24 h later, and then rats were euthanized. The plasma levels of several markers [amylase, diamine oxidase (DAO), fluorescein isothiocyanate (FITC)-dextran, tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-1β, IL-12, lipopolysaccharide (LPS)] and fecal butyric acid level were determined. The pancreas and intestine were examined using histology, and RT-PCR and Western blotting of intestinal tissues were used to measure the expression of six markers {tight junction proteins [zonula occludens protein-1 (ZO-1), claudin-1, claudin-2, occluding], matrix metalloproteinase 9 [MMP9], and TNF-α}. The gut flora of the rats was examined by 16S rRNA sequencing. Induction of SAP + IAH altered several functions of the intestinal barrier, and enhanced intestinal permeability, decreased the levels of ZO-1, claudin-1, occludin, the richness and diversity of the microflora community, the relative abundance (RA) of Firmicutes, and the number of probiotic organisms. However, induction of SAP+IAH increased the expression of claudin-2, MMP9, and TNF-α, and the RA of Proteobacteria and pathogens in the feces. Rats that received oral C. butyricum or butyrate had reduced intestinal injury and plasma levels of DAO, LPS, and inflammatory cytokines. This study of rats with SAP+IAH indicated that oral dosing of C. butyricum or butyrate reduced intestinal injury, possibly by altering the functions of the intestinal mucosal barrier.
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大承气汤减轻重症急性胰腺炎肠血管内皮损伤的体内外研究
影响因子：
--
作者：
Pan, Li-Yun;Chen, Ya-Feng;Feng, Dian-Xu
通讯作者：
Feng, Dian-Xu
影响因子：
7.1
作者：
SCHEPPACH, WM;FABIAN, CE;KASPER, HW
通讯作者：
KASPER, HW
影响因子：
3.7
作者：
Ke L;Tong ZH;Ni HB;Ding WW;Sun JK;Li WQ;Li N;Li JS
通讯作者：
Li JS
影响因子：
3.1
作者：
Kawamura, Takato;Andoh, Akira;Fujiyama, Yoshihide
通讯作者：
Fujiyama, Yoshihide
影响因子：
38.9
作者：
Kirkpatrick, Andrew W.;Roberts, Derek J.;De Waele, Jan;Jaeschke, Roman;Malbrain, Manu L. N. G.;De Keulenaer, Bart;Duchesne, Juan;Bjorck, Martin;Leppaniemi, Ari;Ejike, Janeth C.;Sugrue, Michael;Cheatham, Michael;Ivatury, Rao;Ball, Chad G.;Blaser, Annika Reintam;Regli, Adrian;Balogh, Zsolt J.;D'Amours, Scott;Debergh, Dieter;Kaplan, Mark;Kimball, Edward;Olvera, Claudia
通讯作者：
Olvera, Claudia