Pioneer transcription factors, progesterone receptor and Krüppel like transcription factor 4, cooperatively stimulate the bovine herpesvirus 1 ICP0 early promoter and productive late protein expression.
Pioneer transcription factors, progesterone receptor and Krüppel like transcription factor 4, cooperatively stimulate the bovine herpesvirus 1 ICP0 early promoter and productive late protein expression.
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DOI：
10.1016/j.virusres.2020.198115
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发表时间：
2020-10-15
期刊：
影响因子：
5
通讯作者：
Jones, Clinton
中科院分区：
文献类型：
--
作者：
Sawant, Laximan;Wijesekera, Nishani;Jones, Clinton
关键词：
牛疱疹病毒1型（BoHV-1），包括市售的改良活疫苗，很容易感染胎儿和卵巢，可导致生殖衰竭。感觉神经元中的BoHV-1潜伏-再激活周期进一步使生殖失败复杂化，因为孕酮偶尔诱导潜伏的再激活。孕酮受体（PR）和Krüppel样转录因子15（KLF 15）协同刺激生产性感染和立即早期转录单位1（IEtu 1）启动子。除了IEtu 1启动子外，bICP 0基因还含有一个单独的早期（E）启动子。在这项研究中，我们测试的假设，PR和KLF家族成员反式激活bICP 0 E启动子。PR和KLF 4以协同方式刺激bICP 0 E启动子活性和晚期生产性病毒蛋白表达。进一步的研究表明，bICP 0 E启动子内的三个增强子结构域对PR和KLF 4有反应。染色质免疫沉淀研究表明PR和KLF 4占据bICP 0 E启动子序列在转染的Neuro-2A细胞和牛肾细胞感染后的晚期。免疫共沉淀研究表明PR和KLF 4稳定地相互作用。这些研究表明PR和KLF 4协同激活bICP 0 E启动子与这些先驱转录因子之间的相互作用有关。
Bovine herpesvirus 1 (BoHV-1), including commercially available modified live vaccines, readily infect the fetus and ovaries, which can cause reproductive failure. The BoHV-1 latency-reactivation cycle in sensory neurons further complicates reproductive failure because progesterone sporadically induces reactivation from latency. The progesterone receptor (PR) and Krüppel-like transcription factor 15 (KLF15) cooperatively stimulate productive infection and the immediate early transcription unit 1 (IEtu1) promoter. In addition to the IEtu1 promoter, the bICP0 gene also contains a separate early (E) promoter. In this study, we tested the hypothesis that PR and KLF family members transactivate the bICP0 E promoter. PR and KLF4 stimulated bICP0 E promoter activity and expression of late productive viral protein expression in a cooperative manner. Additional studies revealed three enhancer domains within the bICP0 E promoter were responsive to PR and KLF4. Chromatin immunoprecipitation studies demonstrated PR and KLF4 occupy bICP0 E promoter sequences in transfected Neuro-2A cells and at late times following infection of bovine kidney cells. Co-immunoprecipitation studies indicated PR and KLF4 stably interact with each other. These studies suggest cooperative activation of the bICP0 E promoter by PR and KLF4 correlate with interactions between these pioneer transcription factors.
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DOI：
10.1007/s00018-017-2467-3
发表时间：
2017-06
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Claessens F;Joniau S;Helsen C
通讯作者：
Helsen C
影响因子：
10.5
作者：
Iwafuchi-Doi M;Zaret KS
通讯作者：
Zaret KS
影响因子：
5.4
作者：
El-Mayet, Fouad S.;Sawant, Laximan;Jones, Clinton
通讯作者：
Jones, Clinton
影响因子：
12.3
作者：
Kaczynski J;Cook T;Urrutia R
通讯作者：
Urrutia R
影响因子：
5.4
作者：
da Silva, Leticia Frizzo;Kook, Insun;Jones, Clinton
通讯作者：
Jones, Clinton