Dexamethasone enhances osteogenic differentiation of bone marrow- and muscle-derived stromal cells and augments ectopic bone formation induced by bone morphogenetic protein-2.
Dexamethasone enhances osteogenic differentiation of bone marrow- and muscle-derived stromal cells and augments ectopic bone formation induced by bone morphogenetic protein-2.
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地塞米松可增强骨髓和肌肉源性基质细胞的成骨分化，并增强骨形态发生蛋白 2 诱导的异位骨形成。
DOI：
10.1371/journal.pone.0116462
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Sotome S
中科院分区：
文献类型：
--
作者：
Yuasa M;Yamada T;Taniyama T;Masaoka T;Xuetao W;Yoshii T;Horie M;Yasuda H;Uemura T;Okawa A;Sotome S
我们评估了地塞米松是否增强骨髓源性基质细胞（BMSCs）和肌肉组织源性基质细胞（MuSC）的成骨能力，这两者都被认为有助于骨形态发生蛋白-2（BMP-2）诱导的异位骨形成，并确定了潜在的机制。将大鼠BMSC和MuSC在含有或不含10-7 M地塞米松的生长培养基中培养，然后在含有地塞米松和BMP-2的成骨条件下分化。分析地塞米松对细胞增殖和成骨分化的影响，以及对BMP-2诱导的异位骨形成的影响。地塞米松不仅影响BMSCs和MuSCs的增殖速率，而且影响其亚群组成，并随后在成骨分化过程中增强其成骨能力。在BMP-2的成骨诱导过程中，地塞米松也显著影响BMSC和MuSC中的细胞增殖。在体内异位骨形成模型中，含有地塞米松和BMP-2的肌肉植入支架中的骨形成比仅含有BMP-2的支架中的骨形成高两倍以上。我们的研究结果表明，地塞米松有效地增强BMP-2的成骨能力，并因此可能减少临床应用所需的BMP-2的量，从而减少由过量的BMP-2引起的并发症。 亮点：1.地塞米松诱导具有更高分化潜能的骨髓和肌源性细胞的选择性增殖。2.地塞米松通过改变细胞亚群组成，增强骨髓和肌肉来源细胞的成骨能力。3.地塞米松促进骨形态发生蛋白-2诱导的异位成骨。
We evaluated whether dexamethasone augments the osteogenic capability of bone marrow-derived stromal cells (BMSCs) and muscle tissue-derived stromal cells (MuSCs), both of which are thought to contribute to ectopic bone formation induced by bone morphogenetic protein-2 (BMP-2), and determined the underlying mechanisms. Rat BMSCs and MuSCs were cultured in growth media with or without 10-7 M dexamethasone and then differentiated under osteogenic conditions with dexamethasone and BMP-2. The effects of dexamethasone on cell proliferation and osteogenic differentiation, and also on ectopic bone formation induced by BMP-2, were analyzed. Dexamethasone affected not only the proliferation rate but also the subpopulation composition of BMSCs and MuSCs, and subsequently augmented their osteogenic capacity during osteogenic differentiation. During osteogenic induction by BMP-2, dexamethasone also markedly affected cell proliferation in both BMSCs and MuSCs. In an in vivo ectopic bone formation model, bone formation in muscle-implanted scaffolds containing dexamethasone and BMP-2 was more than two fold higher than that in scaffolds containing BMP-2 alone. Our results suggest that dexamethasone potently enhances the osteogenic capability of BMP-2 and may thus decrease the quantity of BMP-2 required for clinical application, thereby reducing the complications caused by excessive doses of BMP-2. Highlights: 1. Dexamethasone induced selective proliferation of bone marrow- and muscle-derived cells with higher differentiation potential. 2. Dexamethasone enhanced the osteogenic capability of bone marrow- and muscle-derived cells by altering the subpopulation composition. 3. Dexamethasone augmented ectopic bone formation induced by bone morphogenetic protein-2.
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DOI：
10.1016/j.bbrc.2004.06.029
发表时间：
2004-07-30
影响因子：
3.1
作者：
Indrawattana, N;Chen, GP;Bunyaratvej, A
通讯作者：
Bunyaratvej, A
DOI：
10.1016/j.bbrc.2008.12.035
发表时间：
2009-02-06
影响因子：
3.1
作者：
Hayashi, Kumi;Yamaguchi, Toru;Sugimoto, Toshitsugu
通讯作者：
Sugimoto, Toshitsugu
影响因子：
4
作者：
Iu, MF;Kaji, H;Chihara, K
通讯作者：
Chihara, K
影响因子：
4.8
作者：
BELLOWS, CG;WANG, YH;AUBIN, JE
通讯作者：
AUBIN, JE
影响因子：
3
作者：
Boden, SD;Kang, J;Heller, JG
通讯作者：
Heller, JG