Mammospheres from murine mammary stem cell-enriched basal cells: clonal characteristics and repopulating potential.
Mammospheres from murine mammary stem cell-enriched basal cells: clonal characteristics and repopulating potential.
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DOI：
10.1016/j.scr.2013.01.007
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发表时间：
2013-05
影响因子：
1.2
通讯作者：
Sun, Lu-Zhe
中科院分区：
文献类型：
--
作者：
Dong, Qiaoxiang;Wang, Danhan;Bandyopadhyay, Abhik;Gao, Hui;Gorena, Karla M.;Hildreth, Kim;Rebel, Vivienne I.;Walter, Christi A.;Huang, Changjiang;Sun, Lu-Zhe
已经尝试用各种体外和体内测定法鉴定鼠乳腺干细胞（MaSC）。虽然体内再增殖试验仍然是MaSC检测的最确定的试验，但它昂贵、耗时且具有技术挑战性。体外乳腺微球试验被认为是不可靠的，因为主要关注其克隆来源。在目前的研究中，与来自荧光蛋白转基因小鼠的乳腺细胞的共培养实验和延时视频显微镜显示，从分选的基底上皮富集细胞形成的> 90%的乳腺球在干细胞方面是克隆来源的。这些基底细胞衍生的乳腺球在三维细胞外基质培养中在形态上和在体内再增殖测定中在功能上进一步区分。将单个乳腺球或所得三维固体结构移植到无腺体乳腺脂肪垫中，获得了70%的多谱系乳腺重建成功率。因此，这种体外球体形成和分化测定是用于研究MaSC的体内再增殖测定的可靠替代方法。
Identification of murine mammary stem cells (MaSCs) has been attempted with various in vitro and in vivo assays. While, the in vivo repopulation assay remains as the most definitive assay for MaSC detection, it is expensive, time-consuming, and technically challenging. The in vitro mammosphere assay was considered unreliable because of major concerns about its clonal origin. In the current study, co-culture experiments with mammary cells from fluorescent protein transgenic mice and time-lapse video microscopy revealed that > 90% mammospheres formed from sorted basal epithelial-enriched cells were of clonal origin in terms of stem cell. These basal-cell derived mammospheres were further distinguished morphologically in a 3-dimensional extracellular matrix culture and functionally in the in vivo repopulation assay. Transplant of single mammospheres or the resultant 3-dimensional solid structures into gland-free mammary fat pads yielded a 70% success rate of multilineage mammary gland reconstitution. Thus, this in vitro sphere formation and differentiation assay is a reliable alternative to the in vivo repopulation assay for the study of MaSCs.
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影响因子：
10.5
作者：
Dontu, G;Abdallah, WM;Wicha, MS
通讯作者：
Wicha, MS
影响因子：
48
作者：
Reynolds, BA;Rietze, RL
通讯作者：
Rietze, RL
影响因子：
64.8
作者：
Asselin-Labat, Marie-Liesse;Vaillant, Francois;Visvader, Jane E.
通讯作者：
Visvader, Jane E.
影响因子：
64.5
作者：
Guo W;Keckesova Z;Donaher JL;Shibue T;Tischler V;Reinhardt F;Itzkovitz S;Noske A;Zürrer-Härdi U;Bell G;Tam WL;Mani SA;van Oudenaarden A;Weinberg RA
通讯作者：
Weinberg RA
DOI：
10.1007/978-1-61779-815-3_12
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Bandyopadhyay, Abhik;Dong, Qiaoxiang;Sun, Lu-Zhe
通讯作者：
Sun, Lu-Zhe