Expression in Escherichia coli and Purification of Folded rDer p 20, the Arginine Kinase From Dermatophagoides pteronyssinus: A Possible Biomarker for Allergic Asthma.
Expression in Escherichia coli and Purification of Folded rDer p 20, the Arginine Kinase From Dermatophagoides pteronyssinus: A Possible Biomarker for Allergic Asthma.
复制标题
大肠杆菌中的表达和折叠的RDER P 20的纯化，这是来自皮肤pho虫的精氨酸激酶：过敏性哮喘的可能生物标志物。
DOI：
10.4168/aair.2021.13.1.154
复制
发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Valenta R
中科院分区：
文献类型：
--
作者：
Sarzsinszky E;Lupinek C;Vrtala S;Huang HJ;Hofer G;Keller W;Chen KW;Panaitescu CB;Resch-Marat Y;Zieglmayer P;Zieglmayer R;Lemell P;Horak F;Duchêne M;Valenta R
精氨酸激酶（AK）首先在印度餐蛾中被确定为过敏原，随后在各种无脊椎动物和贝类中被证明是过敏原。屋尘螨（HDM）种Dermatophagoides pteronyssinus Der p20中已分离到AK的cDNA编码，但迄今尚未产生和鉴定重组Der p20 （Der p20）变应原。我们报道了重组蛋白在大肠杆菌中的表达。通过尺寸排除色谱法和圆二色光谱法分别纯化了rDer p20，证实其为单体折叠蛋白。使用ak特异性抗体，发现Der p20主要发生在HDM体中，而不发生在粪便颗粒中。30%的临床特征明确的HDM过敏患者（n = 98），其免疫球蛋白E （IgE）反应谱已通过广泛的纯化HDM过敏原（Der f 1、2；Der p 1、2、4、5、7、10、11、14、15、18、21、23和37）测定，对Der p 20表现出IgE反应。IgE对Der p20的反应更常与肺部症状相关。在几种无脊椎动物中检测到AK特异性抗体，Der p20与凡纳滨对虾AK具有IgE交叉反应性。因此，Der p20是一种交叉反应性HDM过敏原，可作为HDM诱导的肺部症状（如哮喘）的诊断标志物。
Arginine kinase (AK) was first identified as an allergen in the Indian-meal moth and subsequently shown to occur as allergen in various invertebrates and shellfish. The cDNA coding for AK from the house dust mite (HDM) species Dermatophagoides pteronyssinus, Der p 20, has been isolated, but no recombinant Der p 20 (rDer p 20) allergen has been produced and characterized so far. We report the expression of Der p 20 as recombinant protein in Escherichia coli. rDer p 20 was purified and shown to be a monomeric, folded protein by size exclusion chromatography and circular dichroism spectroscopy, respectively. Using AK-specific antibodies, Der p 20 was found to occur mainly in HDM bodies, but not in fecal particles. Thirty percent of clinically well-characterized HDM allergic patients (n = 98) whose immunoglobulin E (IgE) reactivity profiles had been determined with an extensive panel of purified HDM allergens (Der f 1, 2; Der p 1, 2, 4, 5, 7, 10, 11, 14, 15, 18, 21, 23 and 37) showed IgE reactivity to Der p 20. IgE reactivity to Der p 20 was more frequently associated with lung symptoms. AKs were detected in several invertebrates with specific antibodies and Der p 20 showed IgE cross-reactivity with AK from shrimp (Litopenaeus vannamei). Thus, Der p 20 is a cross-reactive HDM allergen and may serve as a diagnostic marker for HDM-induced lung symptoms such as asthma.
登录
查看更多内容
DOI：
10.4168/aair.2015.7.5.483
发表时间：
2015-09
期刊：
Allergy, asthma & immunology research
影响因子：
--
作者：
Jeong KY;Lee JY;Son M;Yi MH;Yong TS;Shin JU;Lee KH;Kim YJ;Park KH;Park HJ;Lee JH;Park JW
通讯作者：
Park JW
影响因子：
12.4
作者：
vanRee, R;Antonicelli, L;Bonifazi, F
通讯作者：
Bonifazi, F
影响因子：
2.8
作者：
Liu, Zhigang;Xia, Lixin;Roux, Kenneth H.
通讯作者：
Roux, Kenneth H.
影响因子：
2.2
作者：
Brown, AE;France, RM;Grossman, SH
通讯作者：
Grossman, SH
影响因子：
14.2
作者：
Huang, Huey-Jy;Resch-Marat, Yvonne;Vrtala, Susanne
通讯作者：
Vrtala, Susanne