Role of G protein-coupled estrogen receptor 1, GPER, in inhibition of oocyte maturation by endogenous estrogens in zebrafish.
Role of G protein-coupled estrogen receptor 1, GPER, in inhibition of oocyte maturation by endogenous estrogens in zebrafish.
复制标题
DOI：
10.1016/j.ydbio.2010.03.027
复制
发表时间：
2010-06-15
影响因子：
2.7
通讯作者：
Thomas, Peter
中科院分区：
文献类型：
--
作者：
Pang, Yefei;Thomas, Peter
关键词：
在斑马鱼中研究了雌激素对卵母细胞成熟（OM）的抑制和GPER（以前称为GPR30）的作用。Estradiol-17β (E2)和GPER选择性激动剂G-1结合到斑马鱼卵母细胞膜上，表明GPER存在，免疫细胞化学使用特异性GPER抗体证实了这一点。用芳香化酶抑制剂ATD孵育卵泡封闭卵母细胞，并酶促和人工去除卵巢卵泡细胞层，可显著增加自发性OM，而用100 nM E2或G-1共同处理可部分逆转自发性OM。用GPER抗体孵育脱毛卵母细胞可阻断雌激素对OM的抑制作用，而向卵母细胞微注射雌激素受体α (ERα)反义寡核苷酸则无效。结果表明，卵泡细胞产生的内源性雌激素抑制或延迟斑马鱼卵母细胞的自发成熟，这种雌激素作用是通过GPER介导的。E2和G-1也能减弱硬骨鱼成熟诱导类固醇（17,20β-二羟基-4-孕-3-one， DHP）对OM的刺激作用。E2和G-1下调了DHP诱导OM的中间媒介mPRα的表达。相反，DHP处理导致GPER mRNA水平下降约50%。结果表明，雌激素和GPER是控制斑马鱼OM发病的内分泌系统的关键成分。在硬骨鱼卵母细胞发育的不同阶段，雌激素和孕激素分别通过GPER和mPRα对卵母细胞成熟的开始进行双重控制，提出了一个模型。
Estrogen inhibition of oocyte maturation (OM) and the role of GPER (formerly known as GPR30) were investigated in zebrafish. Estradiol-17β (E2) and G-1, a GPER-selective agonist, bound to zebrafish oocyte membranes suggesting the presence of GPER which was confirmed by immunocytochemistry using a specific GPER antibody. Incubation of follicle-enclosed oocytes with an aromatase inhibitor, ATD, and enzymatic and manual removal of the ovarian follicle cell layers significantly increased spontaneous OM which was partially reversed by co-treatment with either 100 nM E2 or G-1. Incubation of denuded oocytes with the GPER antibody blocked the inhibitory effects of estrogens on OM, whereas microinjection of estrogen receptor alpha (ERα) antisense oligonucleotides into the oocytes was ineffective. The results suggest that endogenous estrogens produced by the follicle cells inhibit or delay spontaneous maturation of zebrafish oocytes and that this estrogen action is mediated through GPER. Treatment with E2 and G-1 also attenuated the stimulatory effect of the teleost maturation-inducing steroid, 17,20β-dihyroxy-4-pregnen-3-one (DHP), on OM. Moreover, E2 and G-1 down-regulated the expression of membrane progestin receptor alpha (mPRα), the intermediary in DHP induction of OM. Conversely DHP treatment caused a > 50% decline in GPER mRNA levels. The results suggest that estrogens and GPER are critical components of the endocrine system controlling the onset of OM in zebrafish. A model is proposed for the dual control of the onset of oocyte maturation in teleosts by estrogens and progestins acting through GPER and mPRα, respectively, at different stages of oocyte development.
登录
查看更多内容
影响因子：
2.7
作者：
Hinckley, M;Vaccari, S;Conti, M
通讯作者：
Conti, M
影响因子：
4.8
作者：
KIRKLAND, JL;MURTHY, L;STANCEL, GM
通讯作者：
STANCEL, GM
影响因子：
2
作者：
KADMON, G;YARON, Z;GORDIN, H
通讯作者：
GORDIN, H
影响因子：
2.7
作者：
Maller, JL
通讯作者：
Maller, JL
影响因子：
4.8
作者：
Motola, Shmulik;Popliker, Malka;Tsafriri, Alex
通讯作者：
Tsafriri, Alex