In vivo quantitative imaging of tumor pH by nanosonophore assisted multispectral photoacoustic imaging.
In vivo quantitative imaging of tumor pH by nanosonophore assisted multispectral photoacoustic imaging.
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DOI：
10.1038/s41467-017-00598-1
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发表时间：
2017-09-07
影响因子：
16.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Jo J;Lee CH;Kopelman R;Wang X
生理pH的变化与多种病理相关，因此开发更有效的医学pH成像方法至关重要。在这里，我们报告在体内pH映射纳米技术。这种表面下化学成像基于肿瘤靶向的pH传感纳米探针和多波长光声成像（派）。该纳米技术包括一种光学pH指示剂SNARF-5 F，5-（和-6）-羧酸，封装在聚丙烯酰胺纳米颗粒中，表面改性用于肿瘤靶向。通过多波长派加上光谱解混技术，生物环境内pH测量的准确性不受生物分子的背景光吸收的影响，即，血红蛋白因此，可以在体内癌症模型中以高灵敏度和高空间分辨率定量评估整个肿瘤的pH水平和血液动力学特性。这里报道的成像技术具有研究和临床管理各种癌症的潜力。几种生物分子的背景光吸收阻碍了肿瘤中有效的体内pH成像。在这里，作者开发了一种基于可见光的体内pH映射方法，通过耦合光声成像和选择性靶向肿瘤细胞的pH响应改性纳米颗粒。
Changes of physiological pH are correlated with several pathologies, therefore the development of more effective medical pH imaging methods is of paramount importance. Here, we report on an in vivo pH mapping nanotechnology. This subsurface chemical imaging is based on tumor-targeted, pH sensing nanoprobes and multi-wavelength photoacoustic imaging (PAI). The nanotechnology consists of an optical pH indicator, SNARF-5F, 5-(and-6)-Carboxylic Acid, encapsulated into polyacrylamide nanoparticles with surface modification for tumor targeting. Facilitated by multi-wavelength PAI plus a spectral unmixing technique, the accuracy of pH measurement inside the biological environment is not susceptible to the background optical absorption of biomolecules, i.e., hemoglobins. As a result, both the pH levels and the hemodynamic properties across the entire tumor can be quantitatively evaluated with high sensitivity and high spatial resolution in in vivo cancer models. The imaging technology reported here holds the potential for both research on and clinical management of a variety of cancers. Background optical absorption of several biomolecules impedes an effective in vivo pH imaging in tumors. Here, the authors developed a visible light-based in vivo pH mapping method by coupling photoacoustic imaging and pH-responsive modified nanoparticles that selectively target tumor cells.
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DOI：
10.1158/1078-0432.ccr-15-0314
发表时间：
2015-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Kimbrough CW;Khanal A;Zeiderman M;Khanal BR;Burton NC;McMasters KM;Vickers SM;Grizzle WE;McNally LR
通讯作者：
McNally LR
影响因子：
3.4
作者：
Berezin, Mikhail Y.;Guo, Kevin;Achilefu, Samuel
通讯作者：
Achilefu, Samuel
影响因子：
64.5
作者：
Hsu, Peggy P.;Sabatini, David M.
通讯作者：
Sabatini, David M.
DOI：
10.1073/pnas.1608247113
发表时间：
2016-07-19
影响因子：
11.1
作者：
Anderson, Michael;Moshnikova, Anna;Andreev, Oleg A.
通讯作者：
Andreev, Oleg A.
影响因子：
3.5
作者：
Jain, R.;Ellika, S. K.;Mikkelsen, T.
通讯作者：
Mikkelsen, T.