D-type cyclins control cell division and developmental rate during Arabidopsis seed development.
D-type cyclins control cell division and developmental rate during Arabidopsis seed development.
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DOI：
10.1093/jxb/ers015
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发表时间：
2012-06
影响因子：
6.9
通讯作者：
Murray JA
中科院分区：
文献类型：
--
作者：
Collins C;Dewitte W;Murray JA
拟南芥种子发育的特点是刻板的分裂模式，这表明细胞周期的协调控制可能需要正确的图案和胚胎和胚乳的生长。D型细胞周期蛋白（CYCD）是细胞周期的重要调控因子，在种子发育过程中发挥重要作用，但对它们参与种子发育的认识仍然有限。在这里，一个家族的基因表达，和损失和获得的功能的方法，通过揭示额外的功能CYCD在种子组织的发展。GUS报告基因的表达表明，CYCD基因在种子中具有离散和重叠的组织特异性表达模式。不同的突变体组合的分析表明，正确的CYCD水平需要在种子发育。CYCD 3亚组是特别需要的，因为它的损失引起发育延迟，而在胚胎和胚乳中过表达CYCD 3;1或先前未表征的基因CYCD 7;1，不同地导致诱导增殖，异常表型，和种子败育增加。CYCD 3; 1的过度表达引起胚胎发育进程的延迟和异常，包括垂体和胚柄的额外分裂，CYCD 3基因正常表达的区域，但不影响胚乳发育。CYCD 7的过表达; 1，在种子发育中不正常表达，通过增加分裂和细胞扩大促进胚和胚乳的过度生长。相反，发芽后的增长，其中模式和器官大小一般不与分裂，结果表明，通过调节CYCD活性的细胞分裂的密切控制是重要的，在种子发育过程中，赋予发育速率和正确的图案。
Seed development in Arabidopsis is characterized by stereotypical division patterns, suggesting that coordinated control of cell cycle may be required for correct patterning and growth of the embryo and endosperm. D-type cyclins (CYCD) are key cell cycle regulators with roles in developmental processes, but knowledge regarding their involvement in seed development remains limited. Here, a family-wide gene expression, and loss- and gain-of-function approach was adopted to reveal additional functions for CYCDs in the development of seed tissues. CYCD genes have both discrete and overlapping tissue-specific expression patterns in the seed as revealed by GUS reporter gene expression. Analysis of different mutant combinations revealed that correct CYCD levels are required in seed development. The CYCD3 subgroup is specifically required as its loss caused delayed development, whereas overexpression in the embryo and endosperm of CYCD3;1 or a previously uncharacterized gene, CYCD7;1, variously leads to induced proliferation, abnormal phenotypes, and elevated seed abortion. CYCD3;1 overexpression provoked a delay in embryonic developmental progression and abnormalities including additional divisions of the hypophysis and suspensor, regions where CYCD3 genes are normally expressed, but did not affect endosperm development. Overexpression of CYCD7;1, not normally expressed in seed development, promoted overgrowth of both embryo and endosperm through increased division and cell enlargement. In contrast to post-germination growth, where pattern and organ size is not generally related to division, results suggest that a close control of cell division through regulation of CYCD activity is important during seed development in conferring both developmental rate and correct patterning.
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影响因子：
7.2
作者：
Colón-Carmona, A;You, R;Doerner, P
通讯作者：
Doerner, P
影响因子：
11.6
作者：
Hutchison, Claire E.;Li, Jie;Kieber, Joseph J.
通讯作者：
Kieber, Joseph J.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1073/pnas.0507581102
发表时间：
2005-10-25
影响因子：
11.1
作者：
Masubelele, NH;Dewitte, W;Murray, JAH
通讯作者：
Murray, JAH
影响因子：
11.6
作者：
Dewitte, W;Riou-Khamlichi, C;Murray, JAH
通讯作者：
Murray, JAH