Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids.
Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids.
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DOI：
10.1186/1471-2229-9-66
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发表时间：
2009-05-29
影响因子：
5.3
通讯作者：
Barro F
中科院分区：
文献类型：
--
作者：
Gil-Humanes J;Pistón F;Martín A;Barro F
APETALA2 样基因形成了一个大型的多基因转录因子家族，在植物生命周期中发挥着重要作用，是许多发育过程的关键调节因子。拟南芥中的许多研究表明，APETALA2 (AP2) 基因参与花分生组织和花器官特性的建立以及花同源异型基因表达的时间和空间调控。在这项工作中，我们分别从大麦和大麦、野生大麦和驯化大麦的种质中克隆和鉴定了 AP2 样基因，并与其他 AP2 同源基因（包括小麦中的 Q 基因）进行了比较。大麦 AP2 样基因包含两个植物特异性 DNA 结合基序，称为 AP2 结构域，小麦的 Q 基因也是如此。我们确认 H. chilense AP2 样基因位于染色体 5Hch 上。在大麦、小麦和三倍体三倍体（大麦×小麦杂交种）的花器官和其他组织中检查了 AP2 样基因的表达模式。在三倍体海龟中，大麦 AP2 样基因的转录水平低于其大麦亲本，并且发现染色体取代 1D/1Hch 和 2D/2Hch 改变了 AP2 基因的表达水平。这些结果对于了解 AP2 样基因在大麦和小麦穗形态中的作用，以及了解该基因在大麦-小麦杂交获得的双倍体中的调控具有重要意义。该信息可应用于谷物育种计划，以上调或下调 AP2 样基因的表达，从而改变穗特征并获得自由脱粒植物。
The APETALA2-like genes form a large multi-gene family of transcription factors which play an important role during the plant life cycle, being key regulators of many developmental processes. Many studies in Arabidopsis have revealed that the APETALA2 (AP2) gene is implicated in the establishment of floral meristem and floral organ identity as well as temporal and spatial regulation of flower homeotic gene expression. In this work, we have cloned and characterised the AP2-like gene from accessions of Hordeum chilense and Hordeum vulgare, wild and domesticated barley, respectively, and compared with other AP2 homoeologous genes, including the Q gene in wheat. The Hordeum AP2-like genes contain two plant-specific DNA binding motifs called AP2 domains, as does the Q gene of wheat. We confirm that the H. chilense AP2-like gene is located on chromosome 5Hch. Patterns of expression of the AP2-like genes were examined in floral organs and other tissues in barley, wheat and in tritordeum amphiploids (barley × wheat hybrids). In tritordeum amphiploids, the level of transcription of the barley AP2-like gene was lower than in its barley parental and the chromosome substitutions 1D/1Hch and 2D/2Hch were seen to modify AP2 gene expression levels. The results are of interest in order to understand the role of the AP2-like gene in the spike morphology of barley and wheat, and to understand the regulation of this gene in the amphiploids obtained from barley-wheat crossing. This information may have application in cereal breeding programs to up- or down-regulate the expression of AP2-like genes in order to modify spike characteristics and to obtain free-threshing plants.
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影响因子：
2
作者：
Atienza, SG;Ramírez, CM;Martín, A
通讯作者：
Martín, A
影响因子：
5.4
作者：
Hernández, P;Dorado, G;Martín, A
通讯作者：
Martín, A
影响因子：
5.4
作者：
Kato, K;Miura, H;Sawada, S
通讯作者：
Sawada, S
影响因子：
64.5
作者：
DREWS, GN;BOWMAN, JL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
11.6
作者：
Klucher, KM;Chow, H;Fischer, RL
通讯作者：
Fischer, RL