Inactivation of two SARS-CoV-2 virus surrogates by electron beam irradiation on large yellow croaker slices and their packaging surfaces.
Inactivation of two SARS-CoV-2 virus surrogates by electron beam irradiation on large yellow croaker slices and their packaging surfaces.
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电子束照射大黄鱼片及其包装表面灭活两种 SARS-CoV-2 病毒替代物
DOI：
10.1016/j.foodcont.2022.109340
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发表时间：
2023-03
期刊：
影响因子：
6
通讯作者：
Xin Wang
中科院分区：
文献类型：
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作者：
Zonghong Luo;Ke Ni;Yuancheng Zhou;Guanhong Chang;Jiangtao Yu;Chunling Zhang;Wenqi Yin;Dishi Chen;Shuwei Li;Shengyao Kuang;Peng Zhang;Kui Li;Junqing Bai;Xin Wang
在与冷链相关的食品和食品包装中检测到传染性SARS-CoV-2，引起了人们对通过冷链物流运输的食品中严重急性呼吸综合征冠状病毒2(SARS-CoV-2)可能的传播途径以及对新的净化策略的需求的关注。本文研究了电子束辐照对猪流行性腹泻病毒(PEDV)和猪传染性胃肠炎病毒(TGEV)这两种SARS-CoV-2代用品在培养基和食物基质中的灭活效果以及对食物基质的影响。用透射电子显微镜和实时定量聚合酶链式反应分析了病毒灭活的原因。在冷藏(0℃)和冷冻(−18℃)条件下，用电子束辐照(2、4、6、8、10kGy)对内外包装的样品进行辐照，包括接种病毒的大黄鱼和病毒悬液。随着电子束照射剂量的增加，悬浮液和FISH中的病毒滴度均显著下降(P<0.05)。两种病毒在悬浮液和鱼体中的最大D10值均为1.24kGy.通过对SARS-CoV-2代理病毒的透射电子显微镜观察和薄片阴性染色，发现剂量低于10kGY的电子束照射可以破坏两种SARS-CoV-2代理病毒的刺突蛋白，使其不具感染性。通过qRT-PCR还发现，超过10kGy剂量的电子束照射也能降解病毒基因组RNA。在低于10kGy的剂量下，辐照鱼样的颜色、pH、TVB-N、TBARS和感官特性没有显著差异。这些发现表明，电子束辐照有可能被开发为一种有效的非热疗方法，以减少通过冷链食品运输的食品中的SARS-CoV-2污染，从而降低通过冷链感染SARS-CoV-2的风险。
The detection of infectious SARS-CoV-2 in food and food packaging associated with the cold chain has raised concerns about the possible transmission pathway of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in foods transported through cold-chain logistics and the need for novel decontamination strategies. In this study, the effect of electron beam (E-beam) irradiation on the inactivation of two SARS-CoV-2surrogate, viruses porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV), in culture medium and food substrate, and on food substrate were investigated. The causes of virus inactivation were also investigated by transmission electron microscopy (TEM) and Quantitative Real-time PCR (QRT-PCR). Samples packed inside and outside, including virus-inoculated large yellow croaker and virus suspensions, were irradiated with E-beam irradiation (2, 4, 6, 8, 10 kGy) under refrigerated (0 °C)and frozen (−18 °C) conditions. The titers of both viruses in suspension and fish decreased significantly (P < 0.05) with increasing doses of E-beam irradiation. The maximum D10 value of both viruses in suspension and fish was 1.24 kGy. E-beam irradiation at doses below 10 kGy was found to destroy the spike proteins of both SARS-CoV-2 surrogate viruses by transmission electron microscopy (TEM) and negative staining of thin-sectioned specimens, rendering them uninfectious. E-beam irradiation at doses greater than 10 kGy was also found to degrade viral genomic RNA by qRT-PCR. There were no significant differences in color, pH, TVB-N, TBARS, and sensory properties of irradiated fish samples at doses below 10 kGy. These findings suggested that E-beam irradiation has the potential to be developed as an efficient non-thermal treatment to reduce SARS-CoV-2 contamination in foods transported through cold chain foods to reduce the risk of SARS-CoV-2 infection in humans through the cold chain.
影响因子：
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作者：
Singh G;Jorgenson J;Pringle T;Nelson T;Ramamoorthy S
通讯作者：
Ramamoorthy S