Monitoring SARS-CoV-2 decontamination by dry heat and ultraviolet treatment with a swine coronavirus as a surrogate.

Monitoring SARS-CoV-2 decontamination by dry heat and ultraviolet treatment with a swine coronavirus as a surrogate.
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以猪冠状病毒为代表监测干热和紫外线治疗对SARS-CoV-2病毒的净化。

DOI:
10.1016/j.infpip.2020.100103
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发表时间:
2021-03
影响因子:
--
通讯作者:
Ramamoorthy S
Ramamoorthy S
中科院分区:
其他
文献类型:
--
作者:
Singh G;Jorgenson J;Pringle T;Nelson T;Ramamoorthy S

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在SARS-CoV-2大流行期间,迫切需要可靠的方法来验证个人防护装备(PPE)的净化方案,以便在SARS-CoV-2大流行期间重复使用,但由于需要专门的遏制设施来处理病毒,这一需求受到限制。因此,我们在此验证了猪冠状病毒作为替代品的使用,并测试了干热和紫外线(UV)对个人防护用品去污的有效性。将实验污染的N95口罩和医用长袍暴露在60°C下20分钟,在1800 MJ/cm2的UVC下暴露,可使替代病毒减少4个对数并灭活。这项研究为验证PPE后处理方法提供了一种新的选择。
The critical need for reliable methods to validate decontamination protocols for personal protective equipment (PPE) for re-use during the SARS-CoV-2 pandemic is limited by the need for specialized containment facilities to handle the virus. Hence, we have herein validated the use of a swine coronavirus as a surrogate, and tested the effectiveness of dry heat and ultraviolet (UV) rays for PPE decontamination. Exposure of experimentally contaminated N95 masks and hospital gowns to 60°C for 20 min, and UVC at 1800 mJ/cm2 resulted in a 4-log reduction and inactivation of the surrogate virus. This study provides a novel alternative to validate PPE reprocessing methods.
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