Root exudation of phytochemicals in Arabidopsis follows specific patterns that are developmentally programmed and correlate with soil microbial functions.
Root exudation of phytochemicals in Arabidopsis follows specific patterns that are developmentally programmed and correlate with soil microbial functions.
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DOI：
10.1371/journal.pone.0055731
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Vivanco JM
中科院分区：
文献类型：
--
作者：
Chaparro JM;Badri DV;Bakker MG;Sugiyama A;Manter DK;Vivanco JM
植物根系不断地向土壤中分泌化合物，与邻近的生物相互作用，可能是为了在不同的发育阶段获得某些功能优势。因此，已经假设根分泌物中存在的植物化学成分在植物的寿命过程中发生变化。在这里，在体外生长的拟南芥植物的根分泌物收集在不同的发展阶段，并使用GC-MS分析。主成分分析表明，根分泌物的组成在每个发展阶段不同。糖和糖醇的累积分泌水平在早期时间点较高，并在发育过程中下降。相反，氨基酸和酚类物质的累积分泌水平随着时间的推移而增加。参与根分泌物中所代表的化合物的生物合成和运输的基因在根中的表达与根分泌物的模式一致。相关性分析进行了体外根分泌模式与功能能力的根际微生物代谢这些化合物在不同发育阶段的拟南芥生长在自然土壤中。根际mRNA的焦磷酸测序揭示了参与碳水化合物、氨基酸和次生代谢物代谢的微生物功能基因与植物发育特定阶段根释放的相应化合物之间的强相关性（p<0.05）。总之，我们的研究结果表明，根分泌过程中的植物化学物质遵循的发展模式，是遗传编程。
Plant roots constantly secrete compounds into the soil to interact with neighboring organisms presumably to gain certain functional advantages at different stages of development. Accordingly, it has been hypothesized that the phytochemical composition present in the root exudates changes over the course of the lifespan of a plant. Here, root exudates of in vitro grown Arabidopsis plants were collected at different developmental stages and analyzed using GC-MS. Principle component analysis revealed that the composition of root exudates varied at each developmental stage. Cumulative secretion levels of sugars and sugar alcohols were higher in early time points and decreased through development. In contrast, the cumulative secretion levels of amino acids and phenolics increased over time. The expression in roots of genes involved in biosynthesis and transportation of compounds represented in the root exudates were consistent with patterns of root exudation. Correlation analyses were performed of the in vitro root exudation patterns with the functional capacity of the rhizosphere microbiome to metabolize these compounds at different developmental stages of Arabidopsis grown in natural soils. Pyrosequencing of rhizosphere mRNA revealed strong correlations (p<0.05) between microbial functional genes involved in the metabolism of carbohydrates, amino acids and secondary metabolites with the corresponding compounds released by the roots at particular stages of plant development. In summary, our results suggest that the root exudation process of phytochemicals follows a developmental pattern that is genetically programmed.
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DOI：
10.2136/sssaj1974.03615995003800040021x
发表时间：
1974-01-01
影响因子：
2.9
作者：
BEHERA, B;WAGNER, GH
通讯作者：
WAGNER, GH
影响因子：
9.7
作者：
Hamer, U;Marschner, B
通讯作者：
Marschner, B
影响因子：
7.4
作者：
Badri, Dayakar V.;Loyola-Vargas, Victor M.;Vivanco, Jorge M.
通讯作者：
Vivanco, Jorge M.
影响因子：
3.6
作者：
JimenezZurdo, JI;GarciaRodriguez, FM;Toro, N
通讯作者：
Toro, N
DOI：
10.1073/pnas.0808005106
发表时间：
2009-08-25
影响因子：
11.1
作者：
Haudecoeur, E.;Planamentea, S.;Faure, D.
通讯作者：
Faure, D.