Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.013821.

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.013821.
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水稻胚乳发育中皮质区域的双重调节 RNA 运输途径文章、出版日期和引文信息可在 www.plantcell.org/cgi/doi/10.1105/tpc.013821 上找到。

DOI:
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发表时间:
2003
期刊:
The Plant Cell Online
影响因子:
--
通讯作者:
T. Okita
T. Okita
中科院分区:
--
文献类型:
--
作者:
S. Hamada;Keiki Ishiyama;Chotipa Sakulsingharoj;Sang;Yujia Wu;Changlin Wang;Salvinder Singh;Naoko Kawai;J. Messing;T. Okita

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在水稻种子发育过程中,醇溶蛋白和谷蛋白RNAs定位于皮层内质网(ER)的两个亚区,即蛋白体ER和囊泡ER。在报告RNA的3‘非翻译区添加几乎全长的醇溶蛋白序列,将其定位从脑池内质网重定向到蛋白体内质网。对醇溶蛋白RNA序列的缺失分析表明,存在蛋白质小体ER靶向所需的两个部分冗余的顺式元件。然而,将谷蛋白3‘非翻译区添加到蛋白体ER顺式序列中,将RNA定位重定向到脑池ER。这些结果表明,至少存在两条调控的RNA转运途径和一条通往皮质内质网的结构性途径。
Prolamine and glutelin RNAs are localized to two subdomains of the cortical endoplasmic reticulum (ER), the protein body ER and the cisternal ER, in developing rice seeds. The addition of nearly full-length prolamine sequences at the 3′ untranslated region of a reporter RNA redirects its localization from the cisternal ER to the protein body ER. Deletion analysis of prolamine RNA sequences indicates the presence of two partially redundant cis elements required for protein body ER targeting. The addition of glutelin 3′ untranslated region to protein body ER cis sequences, however, redirects RNA localization to the cisternal ER. These results indicate that there are at least two regulated RNA transport pathways as well as a constitutive pathway to the cortical ER.
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