Differential functional effects of biomaterials on dendritic cell maturation.
Differential functional effects of biomaterials on dendritic cell maturation.
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DOI：
10.1016/j.actbio.2012.06.006
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发表时间：
2012-10
影响因子：
9.7
通讯作者：
Babensee JE
中科院分区：
文献类型：
--
作者：
Park J;Babensee JE
评估了用不同生物材料处理树突状细胞（DC）的免疫学结果，以证明组合产品中常用的生物材料诱导的DC表型范围。未成熟DC（iDC）来源于人外周血单核细胞，并用藻酸盐、琼脂糖、壳聚糖、透明质酸（HA）或75：25聚（乳酸-共-乙醇酸）（PLGA）的不同生物材料膜处理，并评估表型功能结果的综合干部。根据用于治疗DC的生物材料膜的类型，观察到DC表型的功能变化的差异水平。与iDC相比，用PLGA或壳聚糖膜处理DC支持DC成熟，具有更高水平的DC同种异体刺激能力、促炎细胞因子释放、CD 80、CD 86、CD 83、HLA-DQ和CD 44表达的表达，以及与iDC相比更低水平的内吞能力。藻酸盐膜以高于iDC的水平诱导DC释放促炎细胞因子。与iDC相比，用HA膜处理的树突状细胞表达较低水平的⑶ 40、⑶ 80、⑶ 86和HLA-DR。它们还表现出低于iDC的水平的内吞能力和CD 44表达，这可能是由于具有高分子量HA的不溶性（交联）形式。有趣的是，用琼脂糖膜处理DC将DC功能表型维持在与iDC相似的水平，除了CD 44表达低于iDC的表达水平。综上所述，这些结果可以为用于免疫调节应用的生物材料提供选择标准，并可以告知组合产品中生物材料对应用所需的相关免疫应答的潜在结果。
The immunological outcome of dendritic cell (DC) treatment with different biomaterials was assessed to demonstrate the range of DC phenotypes induced by biomaterials commonly used in combination products. Immature DCs (iDCs) were derived from human peripheral blood monocytes, and treated with different biomaterial films of alginate, agarose, chitosan, hyaluronic acid (HA), or 75:25 poly(lactic-co-glycolic acid) (PLGA) and a comprehensive cadre of phenotypic functional outcomes were assessed. Differential levels of functional changes of DC phenotype were observed depending on the type of biomaterial films used to treat DCs. Treatment of DCs with PLGA or chitosan films supported DC maturation with higher levels of DC allostimulatory capacity, pro-inflammatory cytokine release, expression of CD80, CD86, CD83, HLA-DQ and CD44 expression as compared to iDCs, and endocytic ability at a level lower compared to iDCs. Alginate film induced pro-inflammatory cytokine release from DCs at levels higher than iDCs,. Dendritic cells treated with HA film expressed lower levels of CD40, CD80, CD86 and HLA-DR as compared to iDCs. They also exhibited endocytic ability and CD44 expression at levels lower than iDCs, possibly due to an insolublized (cross-linked) form with high molecular weight HA. Interestingly, treatment of DCs with agarose film maintained a DC functional phenotype at levels similar to iDCs except for CD44 expression which was lower than expression levels for iDCs. Taken together, these results can provide selection criteria for biomaterials to be used in immunomodulating applications and can inform potential outcomes of biomaterials within combination products on associated immune responses as desired by the application.
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影响因子：
14
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