Interfamilial recombination between viruses led to acquisition of a novel translation-enhancing RNA element that allows resistance breaking.
Interfamilial recombination between viruses led to acquisition of a novel translation-enhancing RNA element that allows resistance breaking.
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DOI：
10.1111/nph.12650
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Truniger V
中科院分区：
文献类型：
--
作者：
Miras M;Sempere RN;Kraft JJ;Miller WA;Aranda MA;Truniger V
许多植物病毒依赖于功能性 RNA 元件（称为 3'-UTR 帽独立翻译增强子 (3'-CITE)）来翻译其 RNA。在这篇手稿中，我们为现有的假设提供了直接证据，即 3′-CITE 是模块化的，并且可以通过自然界的重组进行转移，并且这与所创建的病毒的优势相关。通过表征新鉴定的甜瓜坏死斑病毒（MNSV；Tombusviridae）分离株，该病毒能够克服真核翻译起始因子 4E (eIF4E) 介导的抗性，我们发现它在其 3'-UTR 中包含 55 个核苷酸插入。我们提供了强有力的证据，表明该插入是通过与亚洲葫芦蚜传黄化病毒（CABYV；黄病毒科）的 3'-UTR 进行科间重组而获得的。通过构建嵌合病毒，我们发现这种重组序列负责打破耐药性。对报告基因构建体翻译效率的分析表明，该序列在抗性和易感植物中均作为新型 3'-CITE 发挥作用，对于抗性植物中的翻译控制至关重要。总之，我们表明，来自不同科的两种明确识别的病毒之间的重组事件导致了与功能性RNA元件相对应的序列的转移，从而产生了具有感染其他不敏感宿主的能力的新分离株。
Many plant viruses depend on functional RNA elements, called 3′-UTR cap-independent translation enhancers (3′-CITEs), for translation of their RNAs. In this manuscript we provide direct proof for the existing hypothesis that 3′-CITEs are modular and transferable by recombination in nature, and that this is associated with an advantage for the created virus. By characterizing a newly identified Melon necrotic spot virus (MNSV; Tombusviridae) isolate, which is able to overcome eukaryotic translation initiation factor 4E (eIF4E)-mediated resistance, we found that it contains a 55 nucleotide insertion in its 3′-UTR. We provide strong evidence that this insertion was acquired by interfamilial recombination with the 3′-UTR of an Asiatic Cucurbit aphid-borne yellows virus (CABYV; Luteoviridae). By constructing chimeric viruses, we showed that this recombined sequence is responsible for resistance breaking. Analysis of the translational efficiency of reporter constructs showed that this sequence functions as a novel 3′-CITE in both resistant and susceptible plants, being essential for translation control in resistant plants. In conclusion, we showed that a recombination event between two clearly identified viruses from different families led to the transfer of exactly the sequence corresponding to a functional RNA element, giving rise to a new isolate with the capacity to infect an otherwise non-susceptible host.
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