Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF.
Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF.
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DOI：
10.1016/j.isci.2021.103601
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发表时间：
2022-01-21
期刊：
影响因子：
5.8
通讯作者：
Hioki H
中科院分区：
文献类型：
--
作者：
Furuta T;Yamauchi K;Okamoto S;Takahashi M;Kakuta S;Ishida Y;Takenaka A;Yoshida A;Uchiyama Y;Koike M;Isa K;Isa T;Hioki H
哺乳动物的大脑是由跨越几个数量级的大小组成的，从突触到整个大脑。因此，跨多个空间尺度可视化神经回路的技术（多尺度神经元成像）对于破译全脑连接至关重要。在这里，我们开发了这种技术，通过耦合连续的光学显微镜/电子显微镜（LM/EM）成像与抗戊二醛的组织清除方法，ScaleSF。我们的多尺度神经元成像结合了（1）全脑宏观观察，（2）介观电路映射，（3）微观亚细胞成像，和（4）纳米结构的EM成像，允许从大脑到突触的结构信息的无缝集成。我们将这种技术应用于两个不同物种的三个神经回路，小鼠离纹状体，小鼠胼胝体，和绒猴皮质纹状体投射系统，并成功地同时询问其电路结构和突触连接的有针对性的方式。我们的多尺度神经元成像将通过扩大对象的尺度来显着推进对全脑连通性的理解。光学透明组织中的连续光学显微镜/电子显微镜从整个大脑到单个突触的多尺度神经元成像神经回路结构和突触连接的同时询问放大到连续成像的稀有突触接触成像解剖学;光学成像;神经科学;细胞生物学
The mammalian brain is organized over sizes that span several orders of magnitude, from synapses to the entire brain. Thus, a technique to visualize neural circuits across multiple spatial scales (multi-scale neuronal imaging) is vital for deciphering brain-wide connectivity. Here, we developed this technique by coupling successive light microscopy/electron microscopy (LM/EM) imaging with a glutaraldehyde-resistant tissue clearing method, ScaleSF. Our multi-scale neuronal imaging incorporates (1) brain-wide macroscopic observation, (2) mesoscopic circuit mapping, (3) microscopic subcellular imaging, and (4) EM imaging of nanoscopic structures, allowing seamless integration of structural information from the brain to synapses. We applied this technique to three neural circuits of two different species, mouse striatofugal, mouse callosal, and marmoset corticostriatal projection systems, and succeeded in simultaneous interrogation of their circuit structure and synaptic connectivity in a targeted way. Our multi-scale neuronal imaging will significantly advance the understanding of brain-wide connectivity by expanding the scales of objects. Successive light microscopy/electron microscopy in optically cleared tissues Multi-scale neuronal imaging from the entire brain to individual synapses Simultaneous interrogation of neural circuit structure and synaptic connectivity Zooming-in to scarce synaptic contacts with the successive imaging Imaging anatomy; Optical imaging; Neuroscience; Cell biology
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影响因子：
3.5
作者：
Kubota Y;Sohn J;Kawaguchi Y
通讯作者：
Kawaguchi Y
影响因子：
3.5
作者：
Sonomura T;Furuta T;Nakatani I;Yamamoto Y;Unzai T;Matsuda W;Iwai H;Yamanaka A;Uemura M;Kaneko T
通讯作者：
Kaneko T
对医疗统计信息的自由使用的易于使用的软件“ EZR”的调查。
影响因子：
4.8
作者：
Kanda Y
通讯作者：
Kanda Y
影响因子：
64.8
作者：
Harris JA;Mihalas S;Hirokawa KE;Whitesell JD;Choi H;Bernard A;Bohn P;Caldejon S;Casal L;Cho A;Feiner A;Feng D;Gaudreault N;Gerfen CR;Graddis N;Groblewski PA;Henry AM;Ho A;Howard R;Knox JE;Kuan L;Kuang X;Lecoq J;Lesnar P;Li Y;Luviano J;McConoughey S;Mortrud MT;Naeemi M;Ng L;Oh SW;Ouellette B;Shen E;Sorensen SA;Wakeman W;Wang Q;Wang Y;Williford A;Phillips JW;Jones AR;Koch C;Zeng H
通讯作者：
Zeng H
DOI：
10.1083/jcb.6.1.61
发表时间：
1959-01-01
期刊：
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY
影响因子：
--
作者：
HAMA, K
通讯作者：
HAMA, K