The identification, expression profile, and preliminary characterization of Tsunagi protein from Schistosoma japonicum
The identification, expression profile, and preliminary characterization of Tsunagi protein from Schistosoma japonicum
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日本血吸虫 Tsunagi 蛋白的鉴定、表达谱及初步表征
DOI：
10.1007/s00436-010-1904-0
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发表时间：
2010-05
影响因子：
2
通讯作者：
Lei, Li
中科院分区：
文献类型：
--
作者：
Shen, Jijia;Wang, Xiaonan;Liu, Miao;Zhao, Zhirong;Lei, Li
Tsunagi 是一种进化上保守的蛋白质，是果蝇和秀丽隐杆线虫发育过程中种系分化所必需的。在本文中，我们描述了日本血吸虫 (SjTsunagi) 的 Tsunagi 蛋白的同源物。 氨基酸序列分析显示存在保守的RNA识别基序。 SjTsunagi 基因编码的预测蛋白质长度为 177 个氨基酸，估计分子量为 20 kD。免疫印迹和逆转录 PCR 分析证实 SjTsunagi 蛋白在卵、尾蚴、血吸虫以及成年雌性和成年雄性寄生虫中表达。 Pull-down 和免疫共沉淀实验证实 SjTsunagi 蛋白可以在体外与 SjMago nashi 相互作用。总而言之，这是日本血吸虫 SjTsunagi 基因表达和初步表征分析的第一份报告。
Tsunagi is an evolutionarily conserved protein, which is required for germ line differentiation during the development of Drosophila melanogaster and Caenorhabditis elegans. In this paper, we describe a homologue of the Tsunagi protein from Schistosoma japonicum (SjTsunagi). Amino acid sequence analysis showed the presence of a conserved RNA recognition motif. The predicted protein encoded by SjTsunagi gene is 177 amino acids in length with an estimated molecular mass of 20 kD. Immunoblot and reverse transcription-PCR analysis confirms SjTsunagi protein is expressed in eggs, cercariae, schistosomula, and adult female and adult male parasites. Pull-down and co-immunoprecipitation assay confirms that protein of SjTsunagi can interact with SjMago nashi in vitro. Taken together, this is the first report of the expression and preliminary characterization analysis of the SjTsunagi gene from S. japonicum.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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