KPNA2 promotes migration and invasion in epithelial ovarian cancer cells by inducing epithelial-mesenchymal transition via Akt/GSK-3β/Snail activation.
KPNA2 promotes migration and invasion in epithelial ovarian cancer cells by inducing epithelial-mesenchymal transition via Akt/GSK-3β/Snail activation.
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KPNA2 通过 Akt/GSK-3β/Snail 激活诱导上皮间质转化，促进上皮性卵巢癌细胞的迁移和侵袭
DOI：
10.7150/jca.20879
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发表时间：
2018
影响因子：
3.9
通讯作者：
Zheng M
中科院分区：
文献类型：
--
作者：
Huang L;Zhou Y;Cao XP;Lin JX;Zhang L;Huang ST;Zheng M
背景：在上皮性卵巢癌（EOC）组织中，KPNA 2表达增加。但是，它在疾病中的作用尚不清楚。在此，我们研究KPNA 2参与EOC的机制。方法：构建稳定表达KPNA 2或KPNA 2 shRNA的细胞系。使用相关测定和蛋白质印迹分析来评估KPNA 2过表达和敲低对EOC细胞迁移、侵袭和上皮向间质转化（EMT）的影响。采用免疫印迹和免疫荧光法检测Akt/GSK-3β/Snail信号通路的关键组分。结果如下：KPNA 2过表达增加了EOC细胞（EFO-21和SK-OV 3）的迁移和侵袭;这些细胞也表现出EMT的特征。Akt/GSK-3β/Snail信号通路中的关键蛋白在过表达KPNA 2的细胞中也上调。相反，KPNA 2的敲低有效地抑制了这些EOC细胞的迁移和侵袭。结论：KPNA 2可能通过抑制Akt/GSK-3β/Snail信号通路，抑制EMT，从而减少EOC的迁移和侵袭。
Background: Increased karyopherin alpha 2 (KPNA2) expression has been demonstrated in epithelial ovarian carcinoma (EOC) tissue. However, its role in the disease is not clear. Here, we investigate the mechanism of involvement of KPNA2 in EOC. Methods: Stable cell lines expressing KPNA2, or KPNA2 shRNAs, were constructed. The effects of KPNA2 overexpression and knockdown on EOC cell migration, invasion, and epithelial-to-mesenchymal transition (EMT) were evaluated using relevant assays and western blot analysis. Key components of the Akt/GSK-3β/Snail signaling pathway were detected using western blotting and immunofluorescence. Results: KPNA2 overexpression increased the migration and invasion of EOC cells (EFO-21 and SK-OV3); these cells also exhibited characteristics of EMT. Key proteins in the Akt/GSK-3β/Snail signaling pathway were also upregulated in cells overexpressing KPNA2. In contrast, knockdown of KPNA2 effectively suppressed migration and invasion of these EOC cells. Conclusions: KPNA2 may reduce the migration and invasion of EOC by inhibiting the Akt/GSK-3β/Snail signaling pathway and suppressing EMT.
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