Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.
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DOI：
10.1016/j.str.2018.01.007
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发表时间：
2018-02-06
期刊：
影响因子：
--
通讯作者：
Löw C
中科院分区：
文献类型：
--
作者：
Flayhan A;Mertens HDT;Ural-Blimke Y;Martinez Molledo M;Svergun DI;Löw C
鞘脂激活蛋白衍生的脂质纳米粒（SapNPs）是用于膜蛋白重构的新的替代工具。在这里，我们展示了SapNPs的潜力和优势。我们表明，SapA对SapNP形成具有最低的脂质特异性。这些纳米颗粒是模块化的，并根据脂质和鞘脂蛋白组分的化学计量比提供可调范围的尺寸和组成。它们是稳定的，并表现出脂质双层系统的典型特征。我们的数据表明，SapNPs是多功能的，可以适应各种大小和结构的膜蛋白。使用SapA和各种类型的脂质，我们可以重建不同跨膜横截面积的膜蛋白（从14到56个跨膜α螺旋）。SapNP重建的蛋白质结合其各自的配体和更热稳定的洗涤剂溶解的形式相比。此外，SapNPs以紧密的方式包围膜蛋白，允许在无洗涤剂的环境中使用小角度X射线散射研究小膜蛋白的结构。SapA显示出对SapNP形成的最低脂质特异性SapNP是通用的并且可以适应各种大小和结构的MP SapNP重构的MP比在洗涤剂中更稳定SapNP以紧凑的方式封装MP Saposin衍生的脂质纳米颗粒（SapNP）是用于膜蛋白（MP）重构的替代工具。Flayhan等人表征这些纳米颗粒，表明它们是通用的，可以适应各种尺寸和结构的MP。SapNP重建的MP可以被视为可溶性蛋白质，并用于结构和功能研究。
Saposin-derived lipid nanoparticles (SapNPs) are a new alternative tool for membrane protein reconstitution. Here we demonstrate the potential and advantages of SapNPs. We show that SapA has the lowest lipid specificity for SapNP formation. These nanoparticles are modular and offer a tunable range of size and composition depending on the stoichiometric ratio of lipid and saposin components. They are stable and exhibit features typical of lipid-bilayer systems. Our data suggest that SapNPs are versatile and can adapt to membrane proteins of various sizes and architectures. Using SapA and various types of lipids we could reconstitute membrane proteins of different transmembrane cross-sectional areas (from 14 to 56 transmembrane α helices). SapNP-reconstituted proteins bound their respective ligands and were more heat stable compared with the detergent-solubilized form. Moreover, SapNPs encircle membrane proteins in a compact way, allowing structural investigations of small membrane proteins in a detergent-free environment using small-angle X-ray scattering. SapA shows the lowest lipid specificity for SapNP formation SapNPs are versatile and can adapt to MPs of various sizes and architectures SapNP-reconstituted MPs are more stable than in detergent SapNPs encapsulate MPs in a compact manner Saposin-derived lipid nanoparticles (SapNPs) are an alternative tool for membrane protein (MP) reconstitution. Flayhan et al. characterize these nanoparticles, showing that they are versatile and can adapt to MPs of various sizes and architectures. SapNP-reconstituted MPs can be treated as soluble proteins and used for structural and functional studies.
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DOI：
10.1083/jcb.201108131
发表时间：
2011-12-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Grove J;Marsh M
通讯作者：
Marsh M
影响因子：
15
作者：
Chien CH;Helfinger LR;Bostock MJ;Solt A;Tan YL;Nietlispach D
通讯作者：
Nietlispach D
影响因子：
6.4
作者：
Gimpl K;Klement J;Keller S
通讯作者：
Keller S
DOI：
10.1016/j.str.2017.03.006
发表时间：
2017-05-02
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Aryal P;Jarerattanachat V;Clausen MV;Schewe M;McClenaghan C;Argent L;Conrad LJ;Dong YY;Pike ACW;Carpenter EP;Baukrowitz T;Sansom MSP;Tucker SJ
通讯作者：
Tucker SJ
影响因子：
28.3
作者：
Beckham, Katherine S. H.;Ciccarelli, Luciano;Houben, Edith N. G.
通讯作者：
Houben, Edith N. G.