Autologous serum tears improve corneal nerve density and sensitivity in patients with ocular graft-versus-host disease-associated dry eye disease.
Autologous serum tears improve corneal nerve density and sensitivity in patients with ocular graft-versus-host disease-associated dry eye disease.
复制标题
自体血清泪液可改善眼移植物抗宿主病相关干眼病患者的角膜神经密度和敏感性。
DOI：
10.1016/j.jtos.2023.01.004
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Yin,Jia
中科院分区：
文献类型：
--
作者：
Carreno-Galeano,JimenaTatiana;Johns,LynetteK;Dana,Reza;Yin,Jia
移植物抗宿主病（GVHD）是用于治疗恶性和良性血液病的异基因骨髓或造血干细胞移植的多器官并发症。眼部GVHD（oGVHD）的临床标志是干眼病（DED）[1]。自体血清泪液（AST）已用于治疗重度DED，现在已整合到oGVHD治疗中[2]。关于它们对oGVHD患者的角膜神经和角膜敏感性的影响知之甚少。我们评估了12周AST治疗对oGVHD导致的中度至重度DED患者的体征、症状、角膜敏感性和基底神经密度（SND）的影响。这项单中心、前瞻性、观察性研究得到了马萨诸塞州眼耳机构审查委员会的批准，按照《健康保险携带和责任法案》的要求进行，并遵守《赫尔辛基宣言》的原则。纳入了18-80岁诊断为慢性GVHD并患有中度至重度DED的患者。中度至重度DED定义为至少一个月有DED症状，眼表疾病指数（OSDI）调查评分> 22，至少一只眼睛角膜荧光素染色（CFS）≥ 4，对先前治疗（包括润滑、抗炎药物和泪点阻塞（每只眼睛至少一个泪点））无反应。通过从每例患者的6瓶离心血液（总计约42 ml）中采集血清制备AST。用不含防腐剂的0.9%注射级氯化钠将其稀释至20%，并用0.22 μm过滤器过滤。然后将5 ml AST等分至15 ml瓶中。患者
Graft-versus-host disease (GVHD) is a multi-organ complication from allogeneic bone marrow or hematopoietic stem cell transplantation used to treat malignant and benign hematologic diseases. The clinical hall-mark of ocular GVHD (oGVHD) is dry eye disease (DED)[1]. Autologous serum tears (AST) have been used to treat severe DED and are now integrated into oGVHD treatment [2]. Little is known about their effect on corneal nerves and corneal sensitivity in patients with oGVHD. We evaluated the effects of 12-week AST treatment on signs, symptoms, corneal sensitivity, and sub-basal nerve density (SND) in patients with moderate-to-severe DED resulting from oGVHD.This single-center, prospective, observational study was approved by the Institutional Review Board of the Massachusetts Eye and Ear, conducted in accordance with the requirements of the Health Insurance Portability and Accountability Act, and adhered to the tenets of the Declaration of Helsinki. Patients aged 18–80 years old diagnosed with chronic GVHD who had moderate to severe DED were included. Moderate to severe DED was defined as having symptoms of DED for at least one month, Ocular Surface Disease Index (OSDI) survey score> 22, and corneal fluorescein staining (CFS)≥ 4 in at least one eye that failed to respond to previous treatments including lubrication, anti-inflammatory medications, and punctal occlusion (at least one punctum per eye). AST was prepared by collecting serum from 6 vials (approximately 42 ml total) of centrifuged blood from each patient. It was diluted to 20% with preservative free 0.9% injection quality sodium chloride and filtered with 0.22-μm filters. Five ml AST was then aliquoted into 15 ml bottles. Patients
影响因子：
13.7
作者：
Müller RT;Abedi F;Cruzat A;Witkin D;Baniasadi N;Cavalcanti BM;Jamali A;Chodosh J;Dana R;Pavan-Langston D;Hamrah P
通讯作者：
Hamrah P
通过激光扫描和白光系统对正常和患病角膜的人前角膜结构进行体内共焦显微镜比较
DOI：
--
发表时间：
2009
期刊：
影响因子：
--
作者：
W. Schrems;L. Hoesl;M. Dastjerdi;R. Dana;D. Pavan;P. Hamrah
通讯作者：
P. Hamrah
影响因子：
13.7
作者：
Saboo US;Amparo F;Abud TB;Schaumberg DA;Dana R
通讯作者：
Dana R